catalog number :
MBS025277
products type :
ELISA Kit
products full name :
Canine Growth Differentiation Factor 9 ELISA Kit
products short name :
[Growth Differentiation Factor 9]
other names :
[growth/differentiation factor 9 preproprotein; Growth/differentiation factor 9; growth/differentiation factor 9; GDF-9; growth differentiation factor 9]
products gene name :
[GDF9]
other gene names :
[GDF9; GDF9; GDF-9]
uniprot entry name :
GDF9_HUMAN
specificity :
No significant cross-reactivity or interference between this analyte and analogues is observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Canine Body Fluids And Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 31.2pg/ml-1000pg/ml. Sensitivity: 5.0pg/ml.
other info2 :
Intra-assay Precision: Intra-assay CV (%) are less than 15%. Inter-assay Precision: Inter-assay CV (?) are less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.
products description :
Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determine the level of GDF8 (hereafter termed "analyte") in undiluted original Canine body fluids and tissue homogenates. This kit is NOT suitable for assaying non-biological sources of substances.
ncbi acc num :
NP_005251.1
ncbi gb acc num :
NM_005260.3
ncbi mol weight :
51,444 Da
ncbi pathways :
Ovarian Infertility Genes Pathway (198801); Ovarian Steroidogenesis Pathway (790011); Ovarian Steroidogenesis Pathway (795173)
ncbi summary :
Growth factors synthesized by ovarian somatic cells directly affect oocyte growth and function. Growth differentiation factor-9 (GDF9) is expressed in oocytes and is thought to be required for ovarian folliculogenesis. GDF9 is a member of the transforming growth factor-beta superfamily. [provided by RefSeq, Jul 2008]
uniprot summary :
GDF9: Required for ovarian folliculogenesis. Promotes primordial follicle development. Stimulates granulosa cell proliferation. Promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. Attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells. Altered GDF9 function may be involved in ovarian disorders. Rare variants in GDF9 have been found in patients with premature ovarian failure and mothers of dizygotic twins. Belongs to the TGF-beta family. Protein type: Secreted; Cytokine; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 5q31.1. Cellular Component: extracellular space; cytoplasm. Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding. Biological Process: regulation of apoptosis; BMP signaling pathway; oocyte growth; transforming growth factor beta receptor signaling pathway; regulation of MAPKKK cascade; positive regulation of cell proliferation; female gamete generation; negative regulation of cell growth; cell development
size4 :
10x96-Strip-Wells