ELISA/assay

Rat Tar DNA Binding Protein 43 ELISA Kit

MBS025121

48-Strip-Wells

435 USD

ELISA Kit

Rat Tar DNA Binding Protein 43 ELISA Kit

Tar DNA Binding Protein 43

TDP43

TDP43

Rat

No significant cross-reactivity or interference between Rat TDP43 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 3.12 ng/ml - 100 ng/ml. Sensitivity: 1.0 ng/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TDP43 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat TDP43 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TDP43 and TDP43 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TDP43 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TDP43 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat TDP43 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TDP43 and TDP43 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TDP43 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

130750552

ABO32290.1

TARDBP: DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3 -splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3 UTR. Homodimer. Binds specifically to pyrimidine-rich motifs of TAR DNA and to single stranded TG repeated sequences. Binds to RNA, specifically to UG repeated sequences with a minimun of six contiguous repeats. Interacts with ATNX2; the interaction is RNA-dependent. Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen. 3 isoforms of the human protein are produced by alternative splicing. Protein type: RNA-binding. Chromosomal Location of Human Ortholog: 1p36.22. Cellular Component: nucleoplasm; perichromatin fibrils; cytoplasm; nuclear speck; nucleus. Molecular Function: identical protein binding; protein binding; mRNA 3 -UTR binding; RNA binding; double-stranded DNA binding; nucleotide binding; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; RNA splicing; positive regulation of insulin secretion; positive regulation of transcription from RNA polymerase II promoter; mRNA processing. Disease: Amyotrophic Lateral Sclerosis 10, With Or Without Frontotemporal Dementia

48-Strip-Wells

435 USD

96-Strip-Wells

600