Human Nuclear Factor Erythroid 2-Related Factor 2 ELISA Kit | MyBioSource MBS024775 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Nuclear Factor Erythroid 2-Related Factor 2 ELISA Kit

catalog :

MBS024775

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS024775

products type :

ELISA Kit

products full name :

Human Nuclear Factor Erythroid 2-Related Factor 2 ELISA Kit

products short name :

Nuclear Factor Erythroid 2-Related Factor 2

other names :

nuclear factor erythroid 2-related factor 2 isoform 1; Nuclear factor erythroid 2-related factor 2; nuclear factor erythroid 2-related factor 2; HEBP1; NFE2-related factor 2; NF-E2-related factor 2; nuclear factor erythroid-derived 2-like 2; nuclear factor, erythroid derived 2, like 2; nuclear factor (erythroid-derived 2)-like 2; HEBP1; Nuclear factor, erythroid derived 2, like 2

products gene name :

NRF2

other gene names :

NFE2L2; NFE2L2; NRF2; NRF2; NF-E2-related factor 2

uniprot entry name :

NF2L2_HUMAN

reactivity :

Human

specificity :

No significant cross-reactivity or interference between Human NRF2 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 62.5 pg/ml - 2000 pg/ml. Sensitivity: 10 pg/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NRF2 concentrations in Human serum, plasma and other body fluids. Using Purified Human NRF2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NRF2 and NRF2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NRF2 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

products description :

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NRF2 concentrations in Human serum, plasma and other body fluids. Using Purified Human NRF2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NRF2 and NRF2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NRF2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

20149576

ncbi acc num :

NP_006155.2

ncbi gb acc num :

NM_006164.4

uniprot acc num :

Q16236

ncbi mol weight :

67,827 Da

ncbi pathways :

AhR Pathway (755436); Keap1-Nrf2 Pathway (198777); Oxidative Stress Pathway (198916); Protein Processing In Endoplasmic Reticulum Pathway (167325); Protein Processing In Endoplasmic Reticulum Pathway (167190); Selenium Metabolism And Selenoproteins Pathway (198846)

ncbi summary :

This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

uniprot summary :

NRF2: a transcription factor that regulates basal expression and antioxidant induction of a transcription factor for NAD(P)H:quinone oxidoreductase-1 (NQO1) and other detoxifying genes. Targeted for proteasomal degradation by INrf2. Oxidative stress causes its phosphorylation and release from INrf2. Protein type: Transcription factor; DNA-binding. Chromosomal Location of Human Ortholog: 2q31. Cellular Component: nucleoplasm; centrosome; cytoplasm; plasma membrane; cytosol; chromatin; nucleus. Molecular Function: protein domain specific binding; protein binding; DNA binding; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; positive regulation of blood coagulation; unfolded protein response; regulation of embryonic development; proteasomal ubiquitin-independent protein catabolic process; protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage