ELISA/assay

Hamster Vascular Endothelial Growth Factor A ELISA Kit

MBS024541

48-Strip-Wells

435 USD

ELISA Kit

Hamster Vascular Endothelial Growth Factor A ELISA Kit

Vascular Endothelial Growth Factor A

vascular endothelial growth factor A; Vascular endothelial growth factor A; vascular endothelial growth factor A; VPF; VEGF-A; angiogenic factor; vascular permeability factor; Vascular permeability factor

VEGF-A

VEGFA; VEGFA; VEGF; VEGF; VEGF-A; VPF

VEGFA_SHEEP

Hamster

No significant cross-reactivity or interference between Hamster VEGF-A and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 62.5 pg/ml - 2000 pg/ml. Sensitivity: 10 pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated VEGF-A concentrations in Hamster serum, plasma and other body fluids. Using Purified Hamster VEGF-A antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add VEGF-A and VEGF-A antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of VEGF-A in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated VEGF-A concentrations in Hamster serum, plasma and other body fluids. Using Purified Hamster VEGF-A antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add VEGF-A and VEGF-A antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of VEGF-A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

68341933

NP_001020281.1

NM_001025110.1

P50412

17,247 Da

Function: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin . By similarity. Subunit structure: Homodimer; disulfide-linked. Also found as heterodimer with PGF . By similarity. Sequence similarities: Belongs to the PDGF/VEGF growth factor family.

48-Strip-Wells

435 USD

96-Strip-Wells

600