ELISA/assay

Human Transforming Growth Factor beta3 ELISA Kit

MBS024494

48-Strip-Wells

435 USD

ELISA Kit

Human Transforming Growth Factor beta3 ELISA Kit

Transforming Growth Factor beta3

transforming growth factor beta-3 preproprotein; Transforming growth factor beta-3; transforming growth factor beta-3; TGF-beta-3; transforming growth factor-beta3; transforming growth factor, beta 3

TGFbeta3

TGFB3; TGFB3; TGF-beta3; TGF-beta-3; LAP

TGFB3_CHICK

Human

No significant cross-reactivity or interference between Human TGF?3 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 31.2 pg/ml - 1000 pg/ml. Sensitivity: 5.0 pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TGF?3 concentrations in Human serum, plasma and other body fluids. Using Purified Human TGF?3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TGF?3 and TGF?3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TGF?3 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TGF?3 concentrations in Human serum, plasma and other body fluids. Using Purified Human TGF?3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TGF?3 and TGF?3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TGF?3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

45382047

NP_990785.1

NM_205454.1

P16047

47,078 Da

Cell Cycle Pathway (83960); Cell Cycle Pathway (463); Cytokine-cytokine Receptor Interaction Pathway (119307); Cytokine-cytokine Receptor Interaction Pathway (460); Endocytosis Pathway (102290); Endocytosis Pathway (102181); MAPK Signaling Pathway (199082); MAPK Signaling Pathway (83956); MAPK Signaling Pathway (456); TGF-beta Signaling Pathway (83970)

Function: Involved in embryogenesis and cell differentiation. Subunit structure: Homodimer; disulfide-linked. Subcellular location: Secreted. Post-translational modification: The precursor is cleaved into mature TGF-beta-3 and LAP, which remains non-covalently linked to mature TGF-beta-3 rendering it inactive . By similarity. Sequence similarities: Belongs to the TGF-beta family.

48-Strip-Wells

435 USD

96-Strip-Wells

600