catalog number :
MBS024441
products type :
ELISA Kit
products full name :
Fish Tumor Necrosis Factor alpha ELISA Kit
products short name :
Tumor Necrosis Factor alpha
products gene name :
TNFalpha
specificity :
No significant cross-reactivity or interference between Fish TNF? and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 15.6 pg/ml - 500 pg/ml. Sensitivity: 2.0 pg/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TNF? concentrations in Fish serum, plasma and other body fluids. Using Purified Fish TNF? antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TNF? and TNF? antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TNF? in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
products description :
Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TNF? concentrations in Fish serum, plasma and other body fluids. Using Purified Fish TNF? antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TNF? and TNF? antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TNF? in the samples is then determined by comparing the O.D. of the samples to the standard curve.