Human Matrix Gla Protein ELISA Kit | MyBioSource MBS024150 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Matrix Gla Protein ELISA Kit

catalog :

MBS024150

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS024150

products type :

ELISA Kit

products full name :

Human Matrix Gla Protein ELISA Kit

products short name :

Matrix Gla Protein

other names :

matrix Gla protein; Matrix Gla protein; matrix Gla protein; cell growth-inhibiting gene 36 protein; matrix Gla protein; Cell growth-inhibiting gene 36 protein

products gene name :

MGP

other gene names :

MGP; MGP; NTI; GIG36; MGLAP; MGLAP; MGP

uniprot entry name :

MGP_HUMAN

reactivity :

Human

specificity :

No significant cross-reactivity or interference between Human MGP and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 6.25 ng/ml - 200 ng/ml. Sensitivity: 1.0 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MGP concentrations in Human serum, plasma and other body fluids. Using Purified Human MGP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MGP and MGP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MGP in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MGP concentrations in Human serum, plasma and other body fluids. Using Purified Human MGP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MGP and MGP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MGP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

187591

ncbi acc num :

AAB53765.1

uniprot acc num :

P08493

ncbi mol weight :

12,353 Da

ncbi pathways :

Endochondral Ossification Pathway (198812); Validated Transcriptional Targets Of AP1 Family Members Fra1 And Fra2 Pathway (169349)

ncbi summary :

The protein encoded by this gene is secreted and likely acts as an inhibitor of bone formation. The encoded protein is found in the organic matrix of bone and cartilage. Defects in this gene are a cause of Keutel syndrome (KS). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

uniprot summary :

MGP: Associates with the organic matrix of bone and cartilage. Thought to act as an inhibitor of bone formation. Defects in MGP are the cause of Keutel syndrome (KS). KS is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia. Belongs to the osteocalcin/matrix Gla protein family. Protein type: Secreted; Secreted, signal peptide; Extracellular matrix. Chromosomal Location of Human Ortholog: 12p12.3. Cellular Component: extracellular matrix; proteinaceous extracellular matrix. Molecular Function: protein binding; structural constituent of bone; extracellular matrix structural constituent; calcium ion binding. Biological Process: ossification; cell differentiation; regulation of bone mineralization; cartilage condensation. Disease: Keutel Syndrome

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage