ELISA/assay

Rat Nitrotyrosine ELISA Kit

MBS023877

48-Strip-Wells

435 USD

ELISA Kit

Rat Nitrotyrosine ELISA Kit

Nitrotyrosine

nitric oxide synthase, inducible; Nitric oxide synthase, inducible; nitric oxide synthase, inducible; NOSII; NOS type II; inducible NOS; inducible NO synthase; inducible nitric oxide synthase; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase 2, inducible; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; NOSII; Peptidyl-cysteine S-nitrosylase NOS2

NT

NOS2; NOS2; iNOS; NOS2A; Inducible NOS; iNOS; NOSII

NOS2_BOVIN

Rat

1156

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Urine and other Body Fluids. Detection Range: 0.25 nmol/L ~ 8 nmol/L.

Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Rat NT in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Rat NT concentration. The concentration of Rat NT in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Rat NT in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Rat NT concentration. The concentration of Rat NT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Background: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample.

116003827

NP_001070267.1

NM_001076799.1

Q27995

131,208 Da

Amoebiasis Pathway (167315); Amoebiasis Pathway (167191); Arginine And Proline Metabolism Pathway (84202); Arginine And Proline Metabolism Pathway (323); Calcium Signaling Pathway (84289); Calcium Signaling Pathway (459); Chagas Disease (American Trypanosomiasis) Pathway (147808); Chagas Disease (American Trypanosomiasis) Pathway (147795); Disease Pathway (942846); HIF-1 Signaling Pathway (695207)

Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 . By similarity. Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O. Cofactor: Heme group . By similarity.Binds 1 FAD . By similarity.Binds 1 FMN . By similarity.Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme . By similarity. Enzyme regulation: Regulated by calcium/calmodulin . By similarity. Subunit structure: Homodimer. Binds SLC9A3R1 . By similarity. Sequence similarities: Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.

48-Strip-Wells

435 USD

96-Strip-Wells

600