ELISA/assay

Human Low Density Lipoprotein ELISA Kit

MBS023682

48-Strip-Wells

435 USD

ELISA Kit

Human Low Density Lipoprotein ELISA Kit

Low Density Lipoprotein

low density lipoprotein

LDL

Human

No significant cross-reactivity or interference between Human LDL and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.25 mmol/L - 8 mmol/L. Sensitivity: 0.1 mmol/L.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated LDL concentrations in Human serum, plasma and other body fluids. Using Purified Human LDL antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add LDL and LDL antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LDL in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated LDL concentrations in Human serum, plasma and other body fluids. Using Purified Human LDL antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add LDL and LDL antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LDL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

306411097

ADM85894.1

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]

48-Strip-Wells

435 USD

96-Strip-Wells

600