Human Occludin ELISA Kit | MyBioSource MBS023517 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Occludin ELISA Kit

catalog :

MBS023517

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS023517

products type :

ELISA Kit

products full name :

Human Occludin ELISA Kit

products short name :

Occludin

other names :

occludin isoform b; Occludin; occludin; tight junction protein occludin; occludin

products gene name :

OCLN

other gene names :

OCLN; OCLN; BLCPMG

uniprot entry name :

OCLN_HUMAN

reactivity :

Human

specificity :

No significant cross-reactivity or interference between Human OCLN and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 62.5 ng/ml - 2000 ng/ml. Sensitivity: 10 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated OCLN concentrations in Human serum, plasma and other body fluids. Using Purified Human OCLN antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add OCLN and OCLN antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of OCLN in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated OCLN concentrations in Human serum, plasma and other body fluids. Using Purified Human OCLN antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add OCLN and OCLN antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of OCLN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

327478416

ncbi acc num :

NP_001192184.1

ncbi gb acc num :

NM_001205255.1

uniprot acc num :

Q16625

ncbi mol weight :

59,144 Da

ncbi pathways :

Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494); Pathogenic Escherichia Coli Infection Pathway (83103); Pathogenic Escherichia Coli Infection Pathway (672459); Pathogenic Escherichia Coli Infection Pathway (516); TGF-beta Receptor Signaling Pathway (138024)

ncbi summary :

This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]

uniprot summary :

occludin: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. Interacts with TJP1/ZO1 and with VAPA. Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. Belongs to the ELL/occludin family. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 5q13.1. Cellular Component: tight junction; apicolateral plasma membrane; endocytic vesicle; apical plasma membrane; integral to membrane; plasma membrane; intercellular junction; cytoplasmic vesicle; cytosol; cell junction. Molecular Function: thiopurine S-methyltransferase activity; protein domain specific binding; protein binding; structural molecule activity. Biological Process: methylation; intercellular junction assembly and maintenance; apoptosis; S-adenosylmethionine metabolic process; S-adenosylhomocysteine metabolic process; protein complex assembly; cell structure disassembly during apoptosis. Disease: Band-like Calcification With Simplified Gyration And Polymicrogyria

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage