ELISA/assay

Hamster Creatine Kinase, Muscle ELISA Kit

MBS023361

48-Strip-Wells

435 USD

ELISA Kit

Hamster Creatine Kinase, Muscle ELISA Kit

Creatine Kinase, Muscle

creatine kinase, muscle; Creatine kinase M-type; creatine kinase M-type; creatine kinase M chain; creatine kinase, muscle; Creatine kinase M chain; M-CK

CKM

CKM; CKM; CKMM; M-CK; CKMM

KCRM_HUMAN

Hamster

This kit recognizes recombinant and natural Hamster CKM, no significant cross-reactivity or interference was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Detection Range: 6.25 ng/ml ~ 200 ng/ml. Sensitivity: 1.0 ng/ml.

Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Hamster CKM in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Hamster CKM concentration. The concentration of Hamster CKM in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) are less than 15%. Inter-assay Precision: Inter-assay CV (%) are less than 15%.

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample.

119577741

EAW57337.1

P06732

43,101 Da

Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Creatine Metabolism Pathway (106192); Creatine Pathway (413359); Creatine Pathway (468234); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); Regulation Of Retinoblastoma Protein Pathway (137916); Urea Cycle And Metabolism Of Amino Groups Pathway (198758); Creatine-phosphate Biosynthesis Pathway (545319)

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]

CKM: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family. Protein type: Kinase, other; EC 2.7.3.2; Amino Acid Metabolism - arginine and proline. Chromosomal Location of Human Ortholog: 19q13.32. Cellular Component: cytosol. Molecular Function: creatine kinase activity; ATP binding. Biological Process: phosphocreatine biosynthetic process; phosphorylation; creatine metabolic process

48-Strip-Wells

435 USD

96-Strip-Wells

600