Bovine Perforin ELISA Kit | MyBioSource MBS023165 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Bovine Perforin ELISA Kit

catalog :

MBS023165

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS023165

products type :

ELISA Kit

products full name :

Bovine Perforin ELISA Kit

products short name :

Perforin

other names :

perforin; Perforin-1; perforin-1; cytolysin; lymphocyte pore forming protein; lymphocyte pore-forming protein; perforin 1 (pore forming protein); Cytolysin; Lymphocyte pore-forming protein

products gene name :

PRF

other gene names :

PRF1; PRF1; P1; PFP; FLH2; PFN1; HPLH2; PFP; P1; PFP

uniprot entry name :

PERF_HUMAN

reactivity :

Bovine

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Cell Culture Supernatants, Tissue Homogenate, Urine and other Body Fluids. Detection Range: 31.2 pg/ml ~ 1000 pg/ml.

other info2 :

Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Bovine PRF in the specimen, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the specimens and allow the operator to produce a standard curve of Optical Density versus Bovine PRF concentration. The concentration of Bovine PRF in the specimens is then determined by comparing the O.D. of the specimens to the standard curve. Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Bovine PRF in the specimen, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the specimens and allow the operator to produce a standard curve of Optical Density versus Bovine PRF concentration. The concentration of Bovine PRF in the specimens is then determined by comparing the O.D. of the specimens to the standard curve.

products description :

Background: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of specimens. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the specimen.

ncbi gi num :

189847

ncbi acc num :

AAA60065.1

uniprot acc num :

P14222

ncbi mol weight :

61,377 Da

ncbi pathways :

Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Apoptosis Pathway (198797); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Caspase Cascade In Apoptosis Pathway (137974); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); Graft-versus-host Disease Pathway (83124); Graft-versus-host Disease Pathway (536); IL12 Signaling Mediated By STAT4 Pathway (137936)

ncbi summary :

The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

uniprot summary :

PRF1: Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. Monomer, as sobluble protein. Homooligomer. Oligomerization is required for pore formation. Repressed by contact with target cells. Belongs to the complement C6/C7/C8/C9 family. Protein type: Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 10q22. Cellular Component: membrane; cytoplasmic membrane-bound vesicle; extracellular region; integral to membrane; plasma membrane. Molecular Function: protein binding; calcium ion binding; wide pore channel activity. Biological Process: formation of immunological synapse; apoptosis; cytolysis; defense response to tumor cell; cellular defense response; immune response to tumor cell; defense response to virus; transmembrane transport; protein homooligomerization. Disease: Lymphoma, Non-hodgkin, Familial; Aplastic Anemia; Hemophagocytic Lymphohistiocytosis, Familial, 2

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage