Monkey Fibrinogen ELISA Kit | MyBioSource MBS022981 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Monkey Fibrinogen ELISA Kit

catalog :

MBS022981

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS022981

products type :

ELISA Kit

products full name :

Monkey Fibrinogen ELISA Kit

products short name :

Fibrinogen

other names :

fibrinogen; Fibrinogen gamma-B chain; fibrinogen gamma-B chain; gamma'; fibrinogen, gamma polypeptide; fibrinogen gamma chain

products gene name :

FBG

other gene names :

FGG; FGG; Gamma'

uniprot entry name :

FIBG_BOVIN

reactivity :

Monkey

specificity :

No significant cross-reactivity or interference between Monkey FBG and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 0.25 mg/ml - 8 mg/ml. Sensitivity: 0.1 mg/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated FBG concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey FBG antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add FBG and FBG antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FBG in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated FBG concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey FBG antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add FBG and FBG antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FBG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

350

ncbi acc num :

CAA33562.1

uniprot acc num :

P12799

ncbi mol weight :

50,244 Da

ncbi pathways :

Common Pathway (827720); Complement And Coagulation Cascades Pathway (84312); Complement And Coagulation Cascades Pathway (484); Formation Of Fibrin Clot (Clotting Cascade) Pathway (827717); GRB2:SOS Provides Linkage To MAPK Signaling For Intergrins Pathway (827019); Hemostasis Pathway (827694); Integrin AlphaIIb Beta3 Signaling Pathway (827018); Integrin Cell Surface Interactions Pathway (827017); Platelet Aggregation (Plug Formation) Pathway (827712); Platelet Activation, Signaling And Aggregation Pathway (827704)

uniprot summary :

Function: Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. Subunit structure: Heterohexamer; disulfide linked. Contains 2 sets of 3 non-identical chains (alpha, beta and gamma). The 2 heterotrimers are in head to head conformation with the N-termini in a small central domain . By similarity. Subcellular location: Secreted. Domain: A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure. Post-translational modification: Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers. Sequence similarities: Contains 1 fibrinogen C-terminal domain.

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage