catalog number :
MBS022660
products type :
ELISA Kit
products full name :
Monkey Adrenocorticotropic Hormone ELISA Kit
products short name :
Adrenocorticotropic Hormone
products gene name :
ACTH
specificity :
No significant cross-reactivity or interference between Monkey ACTH and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 6.25 pg/ml - 200 pg/ml. Sensitivity: 1.0 pg/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ACTH concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey ACTH antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ACTH and ACTH antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ACTH in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
products description :
Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ACTH concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey ACTH antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ACTH and ACTH antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ACTH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
CAA00890.1