catalog number :
MBS022425
products type :
ELISA Kit
products full name :
Goat Vascular Endothelial Growth Factor ELISA Kit
products short name :
Vascular Endothelial Growth Factor
products gene name :
VEGF
specificity :
No significant cross-reactivity or interference between Goat VEGF and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 31.2 pg/ml - 1000 pg/ml. Sensitivity: 5.0 pg/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated VEGF concentrations in Goat serum, plasma and other body fluids. Using Purified Goat VEGF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add VEGF and VEGF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of VEGF in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
products description :
Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated VEGF concentrations in Goat serum, plasma and other body fluids. Using Purified Goat VEGF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add VEGF and VEGF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of VEGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.