ELISA/assay

Bovine Calcitonin ELISA Kit

MBS022230

48-Strip-Wells

435 USD

ELISA Kit

Bovine Calcitonin ELISA Kit

Calcitonin

calcitonin isoform CT preproprotein; Calcitonin; calcitonin; calcitonin gene-related peptide 1; katacalcin; calcitonin 1; alpha-type CGRP; calcitonin gene-related peptide I; calcitonin/calcitonin-related polypeptide, alpha; calcitonin-related polypeptide alpha; Calcitonin carboxyl-terminal peptide; CCP; PDN-21

CT

CALCA; CALCA; CT; KC; CGRP; CALC1; CGRP1; CGRP-I; CALC1; CCP

CALC_HUMAN

Bovine

No significant cross-reactivity or interference between Bovine CT and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 50 pg/ml - 1600 pg/ml. Sensitivity: 10 pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CT concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine CT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CT and CT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CT in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CT concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine CT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CT and CT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

76880484

NP_001029124.1

NM_001033952.2

P01258

15,466 Da

Amyloids Pathway (366238); Calcitonin-like Ligand Receptors Pathway (106379); Class B/2 (Secretin Family Receptors) Pathway (106378); Disease Pathway (530764); Endothelin Pathway (672453); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Myometrial Relaxation And Contraction Pathways (198759); Signal Transduction Pathway (477114)

This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Nov 2009]

CALCA: Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Belongs to the calcitonin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11p15.2. Cellular Component: extracellular space; cell soma; cytoplasm; extracellular region; terminal button; nucleus. Molecular Function: identical protein binding; protein binding; calcitonin receptor binding; hormone activity; protein complex binding; receptor binding. Biological Process: negative regulation of osteoclast differentiation; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of interleukin-1 alpha production; vasodilation of artery during baroreceptor response to increased systemic arterial blood pressure; response to pain; positive regulation of vasodilation; G-protein signaling, adenylate cyclase activating pathway; protein amino acid phosphorylation; positive regulation of interleukin-8 production; monocyte chemotaxis; leukocyte adhesion; elevation of cytosolic calcium ion concentration; cell-cell signaling; vasculature development; neuropeptide signaling pathway; negative regulation of smooth muscle contraction; regulation of blood pressure; receptor internalization; positive regulation of cAMP biosynthetic process; negative regulation of ossification; feeding behavior; negative regulation of blood pressure; positive regulation of adenylate cyclase activity; positive regulation of macrophage differentiation; inflammatory response; detection of temperature stimulus involved in sensory perception of pain; negative regulation of bone resorption; negative regulation of neurological process; aging; cytosolic calcium ion homeostasis; regulation of heart rate; adenylate cyclase activation; positive regulation of ossification; regulation of systemic arterial blood pressure by neurological process; G-protein coupled receptor internalization; response to heat; endothelial cell proliferation; regulation of inflammatory response; activation of protein kinase activity; endothelial cell migration; negative regulation of transcription, DNA-dependent; embryo implantation

48-Strip-Wells

435 USD

96-Strip-Wells

600