Hamster Matrix Metalloproteinase 7 ELISA Kit | MyBioSource MBS022159 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Hamster Matrix Metalloproteinase 7 ELISA Kit

catalog :

MBS022159

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS022159

products type :

ELISA Kit

products full name :

Hamster Matrix Metalloproteinase 7 ELISA Kit

products short name :

Matrix Metalloproteinase 7

other names :

matrilysin preproprotein; Matrilysin; matrilysin; matrin; pump-1 protease; uterine matrilysin; uterine metalloproteinase; matrix metalloproteinase-7; matrix metalloproteinase 7 (matrilysin, uterine); matrix metallopeptidase 7 (matrilysin, uterine); Matrin; Matrix metalloproteinase-7; MMP-7; Pump-1 protease; Uterine metalloproteinase

products gene name :

MMP-7

other gene names :

MMP7; MMP7; MMP-7; MPSL1; PUMP-1; MPSL1; PUMP1; MMP-7

uniprot entry name :

MMP7_HUMAN

reactivity :

Hamster

sequence length :

267

specificity :

No significant cross-reactivity or interference between Hamster MMP-7 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 0.25 ng/ml - 8 ng/ml. Sensitivity: 0.1 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MMP-7 concentrations in Hamster serum, plasma and other body fluids. Using Purified Hamster MMP-7 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MMP-7 and MMP-7 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MMP-7 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MMP-7 concentrations in Hamster serum, plasma and other body fluids. Using Purified Hamster MMP-7 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MMP-7 and MMP-7 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MMP-7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4505219

ncbi acc num :

NP_002414.1

ncbi gb acc num :

NM_002423.3

uniprot acc num :

P09237

ncbi mol weight :

29,677 Da

ncbi pathways :

AGE/RAGE Pathway (698754); Activation Of Matrix Metalloproteinases Pathway (576264); Alpha6-Beta4 Integrin Signaling Pathway (198807); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Degradation Pathway (730309); Collagen Formation Pathway (645288); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Matrix Metalloproteinases Pathway (198900); Syndecan-1-mediated Signaling Events Pathway (138046)

ncbi summary :

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal protein domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

uniprot summary :

MMP7: Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase. Belongs to the peptidase M10A family. Protein type: EC 3.4.24.23; Secreted; Protease; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11q22.2. Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region. Molecular Function: zinc ion binding; metalloendopeptidase activity. Biological Process: response to drug; extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; defense response to Gram-positive bacterium; antibacterial peptide biosynthetic process; proteolysis; defense response to Gram-negative bacterium; antibacterial peptide secretion; regulation of cell proliferation

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage