ELISA/assay

Porcine Alkaline Phosphatase ELISA Kit

MBS022002

48-Strip-Wells

435 USD

ELISA Kit

Porcine Alkaline Phosphatase ELISA Kit

Alkaline Phosphatase

alkaline phosphatase; Alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphatase, tissue-nonspecific isozyme; glycerophosphatase; tissue-nonspecific ALP; alkaline phosphomonoesterase; liver/bone/kidney-type alkaline phosphatase; alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; Alkaline phosphatase liver/bone/kidney isozyme

ALP

ALPL; ALPL; HOPS; TNAP; APTNAP; TNSALP; AP-TNAP; AP-TNAP; TNSALP

PPBT_HUMAN

Porcine

No significant cross-reactivity or interference between Porcine ALP and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 15.6 U/L - 500 U/L. Sensitivity: 2.0 U/L.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ALP concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine ALP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ALP and ALP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ALP in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated ALP concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine ALP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ALP and ALP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ALP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

178462

AAB59378.1

P05186

57,305 Da

AGE/RAGE Pathway (698754); BDNF Signaling Pathway (712093); Endochondral Ossification Pathway (198812); Folate Biosynthesis Pathway (83018); Folate Biosynthesis Pathway (404); TNF-alpha/NF-kB Signaling Pathway (198884)

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]

ALPL: This isozyme may play a role in skeletal mineralization. Defects in ALPL are a cause of hypophosphatasia (HOPS). HOPS is an inherited metabolic bone disease characterized by defective skeletal mineralization. Four hypophosphatasia forms are distinguished, depending on the age of onset: perinatal, infantile, childhood and adult type. The perinatal form is the most severe and is almost always fatal. Patients with only premature loss of deciduous teeth, but with no bone disease are regarded as having odontohypophosphatasia (odonto). Defects in ALPL are a cause of hypophosphatasia childhood type (HOPSC). Defects in ALPL are a cause of hypophosphatasia infantile type (HOPSI). Belongs to the alkaline phosphatase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.1.3.1; Phosphatase (non-protein); Motility/polarity/chemotaxis; Membrane protein, GPI anchor; Cofactor and Vitamin Metabolism - folate biosynthesis. Chromosomal Location of Human Ortholog: 1p36.12. Cellular Component: extracellular matrix; extracellular space; membrane; plasma membrane; integral to membrane. Molecular Function: protein binding; pyrophosphatase activity; alkaline phosphatase activity; metal ion binding. Biological Process: response to antibiotic; osteoblast differentiation; response to vitamin D; dephosphorylation; response to glucocorticoid stimulus; reproductive developmental process; response to lipopolysaccharide; skeletal development; endochondral ossification. Disease: Hypophosphatasia, Infantile; Hypophosphatasia, Adult; Hypophosphatasia, Childhood

48-Strip-Wells

435 USD

96-Strip-Wells

600