ELISA/assay

Human Telomerase ELISA Kit

MBS021959

48-Strip-Wells

435 USD

ELISA Kit

Human Telomerase ELISA Kit

Telomerase

telomerase reverse transcriptase isoform 1; Telomerase reverse transcriptase; telomerase reverse transcriptase; telomerase catalytic subunit; telomerase-associated protein 2; telomerase reverse transcriptase; HEST2; Telomerase catalytic subunit; Telomerase-associated protein 2

TE

TERT; TERT; TP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1; EST2; TCS1; TRT; TP2

TERT_HUMAN

Human

1132

No significant cross-reactivity or interference between Human TE and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 3.12U/L-100U/L. Sensitivity: 1.0U/L.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TE concentrations in Human serum, plasma and other body fluids. Using Purified Human TE antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TE and TE antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TE in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TE concentrations in Human serum, plasma and other body fluids. Using Purified Human TE antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TE and TE antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TE in the samples is then determined by comparing the O.D. of the samples to the standard curve.

109633031

NP_937983.2

NM_198253.2

O14746

126,997 Da

Cell Cycle Pathway (530733); Chromosome Maintenance Pathway (161048); Disease Pathway (530764); Extension Of Telomeres Pathway (106541); HIF-1-alpha Transcription Factor Network Pathway (138045); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); IL2 Signaling Events Mediated By PI3K Pathway (137938); Id Signaling Pathway (198871); RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway (1016209)

Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq, Jul 2008]

TERT: telomerase reverse transcriptase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The holoenzyme consists of TERT and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced isoforms have been identified, although the full-length sequence of some variants has not been determined. Protein type: Nucleolus; Transferase; EC 2.7.7.49; DNA-binding. Chromosomal Location of Human Ortholog: 5p15.33. Cellular Component: nucleoplasm; chromosome, telomeric region; PML body; nuclear telomere cap complex; telomerase holoenzyme complex; nucleolus. Molecular Function: telomerase activity; protein binding; protein homodimerization activity; RNA-directed RNA polymerase activity; DNA binding; metal ion binding; telomeric DNA binding; RNA-directed DNA polymerase activity; telomeric template RNA reverse transcriptase activity; tRNA binding; nucleotidyltransferase activity. Biological Process: mitochondrion organization and biogenesis; DNA strand elongation; RNA-dependent DNA replication; positive regulation of nitric-oxide synthase activity; telomere maintenance via telomerase; age-dependent telomere shortening; telomere maintenance; RNA interference, production of siRNA; positive regulation of Wnt receptor signaling pathway. Disease: Pulmonary Fibrosis, Idiopathic; Aplastic Anemia; Dyskeratosis Congenita, Autosomal Dominant, 1

48-Strip-Wells

435 USD

96-Strip-Wells

600