Human Podocalyxin ELISA Kit | MyBioSource MBS021477 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Podocalyxin ELISA Kit

catalog :

MBS021477

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS021477

products type :

ELISA Kit

products full name :

Human Podocalyxin ELISA Kit

products short name :

Podocalyxin

other names :

podocalyxin isoform 1; Podocalyxin; podocalyxin; GCTM-2 antigen; podocalyxin-like protein 1; podocalyxin-like; GCTM-2 antigen; Gp200; Podocalyxin-like protein 1

products gene name :

PCX

other gene names :

PODXL; PODXL; PC; PCLP; Gp200; PCLP-1; PCLP; PCLP1; PC; PCLP-1

uniprot entry name :

PODXL_HUMAN

reactivity :

Human

specificity :

No significant cross-reactivity or interference between Human PCX and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 3.12 ng/ml - 100 ng/ml. Sensitivity: 1.0 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PCX concentrations in Human serum, plasma and other body fluids. Using Purified Human PCX antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PCX and PCX antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PCX in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

products description :

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PCX concentrations in Human serum, plasma and other body fluids. Using Purified Human PCX antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PCX and PCX antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PCX in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

66277202

ncbi acc num :

NP_001018121.1

ncbi gb acc num :

NM_001018111.2

uniprot acc num :

O00592

ncbi mol weight :

58,635 Da

ncbi summary :

This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008]

uniprot summary :

PODXL: Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell- cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR- dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. Belongs to the podocalyxin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral. Chromosomal Location of Human Ortholog: 7q32-q33. Cellular Component: ruffle; extracellular space; integral to plasma membrane; microvillus membrane; lamellipodium; cytoplasm; apical plasma membrane; plasma membrane; lipid raft; filopodium. Molecular Function: protein binding. Biological Process: cell migration; negative regulation of cell adhesion; negative regulation of cell-cell adhesion; cell adhesion; positive regulation of cell-cell adhesion mediated by integrin; leukocyte migration; positive regulation of cell migration; regulation of microvillus biogenesis

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage