ELISA/assay

Chicken Procollagen Type I N-Terminal Propeptide ELISA Kit

MBS021332

48-Strip-Wells

435 USD

ELISA Kit

Chicken Procollagen Type I N-Terminal Propeptide ELISA Kit

Procollagen Type I N-Terminal Propeptide

PINP

Chicken

No significant cross-reactivity or interference between Chicken PINP and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 6.25 ng/ml - 200 ng/ml. Sensitivity: 1.0 ng/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PINP concentrations in Chicken serum, plasma and other body fluids. Using Purified Chicken PINP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PINP and PINP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PINP in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PINP concentrations in Chicken serum, plasma and other body fluids. Using Purified Chicken PINP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PINP and PINP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PINP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

48-Strip-Wells

435 USD

96-Strip-Wells

600