ELISA/assay

Human Orexin A ELISA Kit

MBS021290

96 Strip Wells

600 USD

ELISA Kit

Human Orexin A ELISA Kit

Orexin A

orexin; Orexin; orexin; prepro-orexin; hypocretin (orexin) neuropeptide precursor; Hypocretin; HcrtCleaved into the following 2 chains:Orexin-A; Alternative name(s):; Hypocretin-1; Hcrt1Orexin-B; Alternative name(s):; Hypocretin-2

OX-A

HCRT; HCRT; OX; PPOX; NRCLP1; OX; PPORX; PPOX; Hcrt; Hcrt1; Hcrt2

OREX_HUMAN

Human

131

No significant cross-reactivity or interference between Human OX-A and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Intended Uses: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate OX-A concentrations in Human serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research, not for drug, household, therapeutic or diagnostic applications. !Detection Range: The detection range of this kit is 0.25 ng/ml - 8 ng/ml.

Sensitivity: The sensitivity of this kit is 0.1 ng/ml. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate OX-A concentrations in Human serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research, not for drug, household, therapeutic or diagnostic applications!

4557635

NP_001515.1

NM_001524.1

O43612

13,363 Da

Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!G Alpha (q) Signalling Events Pathway 106043!!GPCR Downstream Signaling Pathway 119548!!GPCR Ligand Binding Pathway 161020!!Gastrin-CREB Signalling Pathway Via PKC And MAPK 645295!!Orexin And Neuropeptides FF And QRFP Bind To Their Respective Receptors Pathway 106362!!Peptide Ligand-binding Receptors Pathway 106358!!Signal Transduction Pathway 477114!!Signaling By GPCR Pathway 106356

This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis. [provided by RefSeq, Jan 2010]

Function: Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity. Subcellular location: Rough endoplasmic reticulum . By similarity. Cytoplasmic vesicle . By similarity. Cell junction synapse . By similarity. Note: Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses . By similarity. Tissue specificity: Abundantly expressed in subthalamic nucleus but undetectable in other brain regions tested (hypothalamus was not tested) and in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas. Post-translational modification: Specific enzymatic cleavages at paired basic residues yield the different active peptides. Involvement in disease: Narcolepsy 1 (NRCLP1) [MIM:161400]: Neurological disabling sleep disorder, characterized by excessive daytime sleepiness, sleep fragmentation, symptoms of abnormal rapid-eye-movement (REM) sleep, cataplexy, hypnagogic hallucinations, and sleep paralysis. Cataplexy is a sudden loss of muscle tone triggered by emotions, which is the most valuable clinical feature used to diagnose narcolepsy. Human narcolepsy is primarily a sporadically occurring disorder but familial clustering has been observed.Note: The disease is caused by mutations affecting the gene represented in this entry. Human narcolepsy is associated with a deficient orexin system. Orexins are absent and/or greatly diminished in the brain and cerebrospinal fluid (CSF) of most narcoleptic patients. Ref.9. Sequence similarities: Belongs to the orexin family.

96 Strip Wells

600 USD