ELISA/assay

Human Creatine Kinase-Bb ELISA Kit

MBS021131

48-Strip-Wells

435 USD

ELISA Kit

Human Creatine Kinase-Bb ELISA Kit

Creatine Kinase-Bb

creatine kinase B-type; Creatine kinase B-type; creatine kinase B-type; B-CK; creatine kinase B chain; B-CK; Creatine kinase B chain

CK-BB

CKB; CKB

KCRB_RABIT

Human

No significant cross-reactivity or interference between Human CK-BB and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 1.56 U/L - 50 U/L. Sensitivity: 0.1 U/L.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CK-BB concentrations in Human serum, plasma and other body fluids. Using Purified Human CK-BB antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CK-BB and CK-BB antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CK-BB in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CK-BB concentrations in Human serum, plasma and other body fluids. Using Purified Human CK-BB antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CK-BB and CK-BB antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CK-BB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

126723525

NP_001075730.1

NM_001082261.1

P00567

42,663 Da

Function: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Catalytic activity: ATP + creatine = ADP + phosphocreatine. Subunit structure: Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. Subcellular location: Cytoplasm. Sequence similarities: Belongs to the ATP:guanido phosphotransferase family.Contains 1 phosphagen kinase C-terminal domain.Contains 1 phosphagen kinase N-terminal domain.

48-Strip-Wells

435 USD

96-Strip-Wells

600