Bovine Vitamin D Binding Protein ELISA Kit | MyBioSource MBS020996 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Bovine Vitamin D Binding Protein ELISA Kit

catalog :

MBS020996

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS020996

products type :

ELISA Kit

products full name :

Bovine Vitamin D Binding Protein ELISA Kit

products short name :

Vitamin D Binding Protein

other names :

vitamin D-binding protein isoform 3; Vitamin D-binding protein; vitamin D-binding protein; VDB; gc-globulin; vitamin D-binding alpha-globulin; group-specific component (vitamin D binding protein); Gc-globulin; Group-specific component

products gene name :

DBP

other gene names :

GC; GC; DBP; GRD3; VDBG; VDBP; DBP/GC; DBP; VDB

uniprot entry name :

VTDB_HUMAN

reactivity :

Bovine

specificity :

No significant cross-reactivity or interference between Bovine DBP and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 6.25 ng/ml - 200 ng/ml. Sensitivity: 1.0 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated DBP concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine DBP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add DBP and DBP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DBP in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated DBP concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine DBP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add DBP and DBP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DBP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

324021745

ncbi acc num :

NP_001191236.1

ncbi gb acc num :

NM_001204307.1

uniprot acc num :

P02774

ncbi mol weight :

52,964 Da

ncbi pathways :

Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Metabolism Of Steroid Hormones And Vitamin D Pathway (106150); Vitamin D (calciferol) Metabolism Pathway (160956); Vitamin D Synthesis Pathway (198764)

ncbi summary :

The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]

uniprot summary :

GC: Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4q12-q13. Cellular Component: extracellular space; lysosomal lumen; extracellular region; cytosol. Molecular Function: vitamin D binding; actin binding; vitamin transporter activity. Biological Process: steroid metabolic process; vitamin transport; vitamin D metabolic process. Disease: Graves Disease, Susceptibility To, 1

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage