ELISA/assay

Rat Thrombopoietin ELISA Kit

MBS020824

48-Strip-Wells

435 USD

ELISA Kit

Rat Thrombopoietin ELISA Kit

Thrombopoietin

thrombopoietin; Thrombopoietin; thrombopoietin; MPL ligand; c-mpl ligand; thrombopoietin nirs variant 1; megakaryocyte stimulating factor; megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; myeloproliferative leukemia virus oncogene ligand; thrombopoietin; C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand

TPO

THPO; THPO; ML; TPO; MGDF; MKCSF; MPLLG; THCYT1; MGDF; ML; MGDF

TPO_HUMAN

Rat

No significant cross-reactivity or interference between Rat TPO and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 15.6 pg/ml - 500 pg/ml. Sensitivity: 2.0 pg/ml.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TPO concentrations in Rat serum, plasma and other body fluids. Using Purified Rat TPO antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TPO and TPO antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TPO in the samples is then determined by comparing the O.D. of the samples to the standard curve.

914226

AAB33390.1

P40225

37,823 Da

Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Hemostasis Pathway (106028); Platelet Aggregation (Plug Formation) Pathway (106053); Platelet Activation, Signaling And Aggregation Pathway (106034)

Megakaryocytopoiesis is the cellular development process that leads to platelet production. The protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternate splicing results in multiple transcript variants of this gene.[provided by RefSeq, Jun 2012]

THPO: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Defects in THPO are the cause of thrombocythemia type 1 (THCYT1). A myeloproliferative disorder characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. Belongs to the EPO/TPO family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Cell cycle regulation; Secreted; Secreted, signal peptide; Cytokine. Chromosomal Location of Human Ortholog: 3q27. Cellular Component: extracellular space. Molecular Function: growth factor activity; hormone activity; cytokine activity. Biological Process: positive regulation of protein kinase B signaling cascade; platelet activation; cell proliferation; multicellular organismal development; myeloid cell differentiation; positive regulation of megakaryocyte differentiation; positive regulation of protein amino acid phosphorylation; blood coagulation. Disease: Thrombocythemia 1

48-Strip-Wells

435 USD

96-Strip-Wells

600