ELISA/assay

Human Thyroxine Binding Globulin ELISA Kit

MBS020751

48-Strip-Wells

435 USD

ELISA Kit

Human Thyroxine Binding Globulin ELISA Kit

Thyroxine Binding Globulin

thyroxine-binding globulin; Thyroxine-binding globulin; thyroxine-binding globulin; serpin A7; T4-binding globulin; thyroxin-binding globulin; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7; Serpin A7; T4-binding globulin

TBG

SERPINA7; SERPINA7; TBG; TBG

THBG_HUMAN

Human

No significant cross-reactivity or interference between Human TBG and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 3.12 ug/ml - 100 ug/ml. Sensitivity: 1.0 ug/ml.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TBG concentrations in Human serum, plasma and other body fluids. Using Purified Human TBG antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TBG and TBG antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TBG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

205277441

NP_000345.2

NM_000354.5

P05543

46,325 Da

There are three proteins including thyroxine-binding globulin (TBG), transthyretin and albumin responsible for carrying the thyroid hormones thyroxine (T4) and 3,5,3'-triiodothyronine (T3) in the bloodstream. This gene encodes the major thyroid hormone transport protein, TBG, in serum. It belongs to the serpin family in genomics, but the protein has no inhibitory function like many other members of the serpin family. Mutations in this gene result in TGB deficiency, which has been classified as partial deficiency, complete deficiency, and excess, based on the level of serum TBG. Alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of these variants has not been determined.[provided by RefSeq, Jun 2012]

SERPINA7: Major thyroid hormone transport protein in serum. Defects in SERPINA7 are a cause of thyroxine-binding globulin deficiency (TBG deficiency). Mutations in the SERPINA7 gene can result as a whole spectrum of deficiencies, characterized by either reduced or increased TBG levels in the serum. Patients show, respectively, reduced or elevated protein- bound iodine but are euthyroid. Belongs to the serpin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: Xq22.2. Cellular Component: extracellular space; extracellular region. Molecular Function: serine-type endopeptidase inhibitor activity; hormone binding. Biological Process: response to drug; response to peptide hormone stimulus; response to vitamin A; aging; post-embryonic development; response to corticosterone stimulus

48-Strip-Wells

435 USD

96-Strip-Wells

600