Human Cytochrome P450 2E1 ELISA Kit | MyBioSource MBS020655 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Cytochrome P450 2E1 ELISA Kit

catalog :

MBS020655

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS020655

products type :

ELISA Kit

products full name :

Human Cytochrome P450 2E1 ELISA Kit

products short name :

Cytochrome P450 2E1

other names :

cytochrome P450 2E1; Cytochrome P450 2E1; cytochrome P450 2E1; CYPIIE1; cytochrome P450-J; microsomal monooxygenase; xenobiotic monooxygenase; 4-nitrophenol 2-hydroxylase; flavoprotein-linked monooxygenase; cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1; cytochrome P450, family 2, subfamily E, polypeptide 1; 4-nitrophenol 2-hydroxylase (EC:1.14.13.n7); CYPIIE1; Cytochrome P450-JCleaved into the following chain:Cytochrome P450 2E1, N-terminally processed

products gene name :

CYP2E1

other gene names :

CYP2E1; CYP2E1; CPE1; CYP2E; P450-J; P450C2E; CYP2E

uniprot entry name :

CP2E1_HUMAN

reactivity :

Human

specificity :

No significant cross-reactivity or interference between this analyte and analogues is observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma and Tissue Homogenate. Detection Range: 0.5 ng/ml - 16 ng/ml. Sensitivity: 0.1 ng/ml. Assay Type: Sandwich

other info2 :

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

products description :

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of CYP2E1 (hereafter termed this analyte) in undiluted original Human serum, plasma and tissue homogenate samples.

ncbi gi num :

10834998

ncbi acc num :

NP_000764.1

ncbi gb acc num :

NM_000773.3

uniprot acc num :

P05181

ncbi mol weight :

56,849 Da

ncbi pathways :

Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (366); Biological Oxidations Pathway (105698); CYP2E1 Reactions Pathway (105705); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Cytochrome P450 - Arranged By Substrate Type Pathway (105700); Drug Metabolism - Cytochrome P450 Pathway (83032); Drug Metabolism - Cytochrome P450 Pathway (427); Fatty Acid Omega Oxidation Pathway (198915)

ncbi summary :

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. [provided by RefSeq, Jul 2008]

uniprot summary :

CYP2E1: Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. Belongs to the cytochrome P450 family. Protein type: EC 1.14.13.n7; Mitochondrial; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Lipid Metabolism - linoleic acid; Lipid Metabolism - arachidonic acid; Xenobiotic Metabolism - metabolism by cytochrome P450; Endoplasmic reticulum; Oxidoreductase. Chromosomal Location of Human Ortholog: 10q26.3. Cellular Component: Golgi membrane; intrinsic to endoplasmic reticulum membrane; endoplasmic reticulum membrane; mitochondrion; intracellular membrane-bound organelle; cytoplasm. Molecular Function: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen; enzyme binding; iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; arachidonic acid epoxygenase activity; steroid hydroxylase activity; oxidoreductase activity; oxygen binding; monooxygenase activity. Biological Process: steroid metabolic process; triacylglycerol metabolic process; response to ethanol; xenobiotic metabolic process; response to ozone; exogenous drug catabolic process; monoterpenoid metabolic process; epoxygenase P450 pathway; heterocycle metabolic process; response to organic nitrogen; drug metabolic process

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage