ELISA/assay

Canine Brain Natriuretic Peptide ELISA Kit

MBS020512

48-Strip-Wells

435 USD

ELISA Kit

Canine Brain Natriuretic Peptide ELISA Kit

Brain Natriuretic Peptide

brain natriuretic peptide

BNP

Canine

No significant cross-reactivity or interference between Canine BNP and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 50 pg/ml - 1600 pg/ml. Sensitivity: 10 pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated BNP concentrations in Canine serum, plasma and other body fluids. Using Purified Canine BNP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add BNP and BNP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of BNP in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated BNP concentrations in Canine serum, plasma and other body fluids. Using Purified Canine BNP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add BNP and BNP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of BNP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4433127

BAA04067.1

This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq, Nov 2014]

48-Strip-Wells

435 USD

96-Strip-Wells

600