Canine alpha Smooth Muscle Actin ELISA Kit | MyBioSource MBS019940 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Canine alpha Smooth Muscle Actin ELISA Kit

catalog :

MBS019940

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS019940

products type :

ELISA Kit

products full name :

Canine alpha Smooth Muscle Actin ELISA Kit

products short name :

alpha Smooth Muscle Actin

other names :

actin, gamma-enteric smooth muscle isoform 1; Actin, gamma-enteric smooth muscle; actin, gamma-enteric smooth muscle; alpha-actin-3; actin-like protein; actin, gamma 2, smooth muscle, enteric; Alpha-actin-3; Gamma-2-actin; Smooth muscle gamma-actin

products gene name :

alpha-SMA

other gene names :

ACTG2; ACTG2; ACT; ACTE; VSCM; ACTA3; ACTL3; ACTSG; ACTA3; ACTL3; ACTSG

uniprot entry name :

ACTH_HUMAN

reactivity :

Canine

sequence length :

376

specificity :

No significant cross-reactivity or interference between Canine alpha-SMA and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 31.2 pg/ml - 1000 pg/ml. Sensitivity: 5.0 pg/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated alpha-SMA concentrations in Canine serum, plasma and other body fluids. Using Purified Canine alpha-SMA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add alpha-SMA and alpha-SMA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of alpha-SMA in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

products description :

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated alpha-SMA concentrations in Canine serum, plasma and other body fluids. Using Purified Canine alpha-SMA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add alpha-SMA and alpha-SMA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of alpha-SMA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4501889

ncbi acc num :

NP_001606.1

ncbi gb acc num :

NM_001615.3

uniprot acc num :

P63267

ncbi mol weight :

41,877 Da

ncbi pathways :

Muscle Contraction Pathway (106261); Smooth Muscle Contraction Pathway (161025); Vascular Smooth Muscle Contraction Pathway (96530); Vascular Smooth Muscle Contraction Pathway (96866)

ncbi summary :

Actins are highly conserved proteins that are involved in various types of cell motility and in the maintenance of the cytoskeleton. Three types of actins, alpha, beta and gamma, have been identified in vertebrates. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. This gene encodes actin gamma 2; a smooth muscle actin found in enteric tissues. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Based on similarity to peptide cleavage of related actins, the mature protein of this gene is formed by removal of two N-terminal peptides.[provided by RefSeq, Dec 2010]

uniprot summary :

ACTG2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Belongs to the actin family. Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Contractile. Chromosomal Location of Human Ortholog: 2p13.1. Cellular Component: extracellular space; cytoskeleton; cytosol. Molecular Function: ATP binding. Biological Process: muscle contraction. Disease: Visceral Myopathy

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage