catalog number :
MBS019698
products type :
ELISA Kit
products full name :
Mouse Heparin-Binding Epidermal Growth Factor Like-Growth Factor ELISA Kit
products short name :
Heparin-Binding Epidermal Growth Factor Like-Growth Factor
other names :
Hb-egf protein, partial
products gene name :
HB-EGF
specificity :
No significant cross-reactivity or interference between Mouse HB-EGF and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 31.2 pg/ml - 1000 pg/ml. Sensitivity: 5.0 pg/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated HB-EGF concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse HB-EGF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add HB-EGF and HB-EGF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HB-EGF in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated HB-EGF concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse HB-EGF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add HB-EGF and HB-EGF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HB-EGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
CCU55305.1