Porcine 5-Lipoxygenase ELISA Kit | MyBioSource MBS019597 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Porcine 5-Lipoxygenase ELISA Kit

catalog :

MBS019597

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS019597

products type :

ELISA Kit

products full name :

Porcine 5-Lipoxygenase ELISA Kit

products short name :

5-Lipoxygenase

other names :

5-lipoxygenase; Arachidonate 5-lipoxygenase; arachidonate 5-lipoxygenase; LOX-5; leukotriene A4 synthase; arachidonic acid 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform; arachidonate 5-lipoxygenase

products gene name :

5-LO

other gene names :

ALOX5; ALOX5; 5-LO; 5LPG; LOG5; 5-LOX; LOG5; 5-LO

uniprot entry name :

LOX5_HUMAN

reactivity :

Porcine

specificity :

No significant cross-reactivity or interference between Porcine 5-LO and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 31.2 U/L - 1000 U/L. Sensitivity: 5.0 U/L.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated 5-LO concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine 5-LO antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add 5-LO and 5-LO antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 5-LO in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

products description :

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated 5-LO concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine 5-LO antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add 5-LO and 5-LO antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 5-LO in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

187167

ncbi acc num :

AAA63212.1

uniprot acc num :

P09917

ncbi mol weight :

77,983 Da

ncbi pathways :

Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553); Arachidonic Acid Metabolism Pathway (366); Eicosanoid Synthesis Pathway (198888); IL-5 Signaling Pathway (198770); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Ovarian Steroidogenesis Pathway (790011); Ovarian Steroidogenesis Pathway (795173); Selenium Pathway (198825)

ncbi summary :

This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

uniprot summary :

5-LO: arachidonate 5-lipoxygenase. Arachidonic acid promotes its phosphorylation by p38 MAPK-regulated MAPKAP kinases. Upregulated in glioblastoma multiforme and colon, pancreatic and bladder cancer. Protein type: EC 1.13.11.34; Lipid Metabolism - arachidonic acid; Nuclear envelope; Lipid Metabolism - linoleic acid; Oxidoreductase. Chromosomal Location of Human Ortholog: 10q11.2. Cellular Component: extracellular space; nuclear membrane; nuclear matrix; nuclear envelope lumen; nuclear envelope; cytosol. Molecular Function: protein binding; iron ion binding; arachidonate 5-lipoxygenase activity. Biological Process: leukotriene biosynthetic process; leukotriene production during acute inflammatory response; lipoxygenase pathway; arachidonic acid metabolic process; leukotriene metabolic process. Disease: Asthma, Susceptibility To

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage