ELISA/assay

Rat Sodium/Glucose Cotransporter 1 ELISA Kit

MBS019321

96 Strip Wells

600 USD

ELISA Kit

Rat Sodium/Glucose Cotransporter 1 ELISA Kit

Sodium/Glucose Cotransporter 1

sodium/glucose cotransporter 1 isoform 1; Sodium/glucose cotransporter 1; sodium/glucose cotransporter 1; Na+/glucose cotransporter 1; high affinity sodium-glucose cotransporter; solute carrier family 5 (sodium/glucose cotransporter), member 1; High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1

SGLT1

SLC5A1; SLC5A1; NAGT; SGLT1; D22S675; NAGT; SGLT1; Na(+)/glucose cotransporter 1

SC5A1_HUMAN

Rat

No significant cross-reactivity or interference between Rat SGLT1 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SGLT1 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat SGLT1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SGLT1 and SGLT1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SGLT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Detection Range: The detection range of this kit is 0.25 ng/ml - 8 ng/ml.

Sensitivity: The sensitivity of this kit is 0.1 ng/ml. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SGLT1 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat SGLT1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SGLT1 and SGLT1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SGLT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4507031

NP_000334.1

NM_000343.3

P13866

73,498 Da

Bile Secretion Pathway 193146!!Bile Secretion Pathway 193095!!Carbohydrate Digestion And Absorption Pathway 170720!!Carbohydrate Digestion And Absorption Pathway 170654!!Hexose Transport Pathway 106198!!Metabolism Pathway 477135!!Metabolism Of Carbohydrates Pathway 106196!!Mineral Absorption Pathway 212237!!Mineral Absorption Pathway 212220!!Na+-dependent Glucose Transporters Pathway 119574

This gene encodes a member of the sodium-dependent glucose transporter (SGLT) family. The encoded integral membrane protein is the primary mediator of dietary glucose and galactose uptake from the intestinal lumen. Mutations in this gene have been associated with glucose-galactose malabsorption. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Function: Actively transports glucose into cells by Na+ cotransport with a Na+ to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na+/glucose cotransporter arranged in series along kidney proximal tubules. Subcellular location: Membrane; Multi-pass membrane protein. Tissue specificity: Expressed mainly in intestine and kidney. Post-translational modification: N-glycosylation is not necessary for the cotransporter function. Involvement in disease: Congenital glucose/galactose malabsorption (GGM) [MIM:606824]: Intestinal monosaccharide transporter deficiency. It is an autosomal recessive disorder manifesting itself within the first weeks of life. It is characterized by severe diarrhea and dehydration which are usually fatal unless glucose and galactose are eliminated from the diet.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.2 Ref.8 Ref.9 Ref.10. Sequence similarities: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. [View classification]

96 Strip Wells

600 USD