Canine Complement Factor D ELISA Kit | MyBioSource MBS018970 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Canine Complement Factor D ELISA Kit

catalog :

MBS018970

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS018970

products type :

ELISA Kit

products full name :

Canine Complement Factor D ELISA Kit

products short name :

Complement Factor D

other names :

complement factor D preproprotein; Complement factor D; complement factor D; properdin factor D; C3 convertase activator; complement factor D preproprotein; D component of complement (adipsin); complement factor D (adipsin); Adipsin; C3 convertase activator; Properdin factor D

products gene name :

CFD

other gene names :

CFD; CFD; DF; ADN; PFD; ADIPSIN; DF; PFD

uniprot entry name :

CFAD_HUMAN

reactivity :

Canine

specificity :

No significant cross-reactivity or interference between Canine CFD and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 0.25 ug/ml - 8 ug/ml. Sensitivity: 0.1 ug/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CFD concentrations in Canine serum, plasma and other body fluids. Using Purified Canine CFD antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CFD and CFD antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CFD in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CFD concentrations in Canine serum, plasma and other body fluids. Using Purified Canine CFD antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CFD and CFD antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CFD in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

42544239

ncbi acc num :

NP_001919.2

ncbi gb acc num :

NM_001928.2

uniprot acc num :

P00746

ncbi mol weight :

27,033 Da

ncbi pathways :

Adipogenesis Pathway (198832); Alternative Complement Activation Pathway (106410); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (106405); Hemostasis Pathway (106028); Immune System Pathway (106386); Initial Triggering Of Complement Pathway (106406); Innate Immune System Pathway (106387); Platelet Activation, Signaling And Aggregation Pathway (106034)

ncbi summary :

The protein encoded by this gene is a member of the trypsin family of peptidases. The encoded protein is a component of the alternative complement pathway best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. [provided by RefSeq, Jul 2008]

uniprot summary :

CFD: Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. Defects in CFD are the cause of complement factor D deficiency (CFDD). CFDD is an immunologic disorder characterized by increased susceptibility to bacterial infections, particularly Neisseria infections, due to a defect in the alternative complement pathway. Belongs to the peptidase S1 family. Protein type: Secreted, signal peptide; Protease; EC 3.4.21.46; Secreted. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: extracellular space; extracellular region. Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity. Biological Process: platelet activation; platelet degranulation; complement activation, alternative pathway; innate immune response; blood coagulation; proteolysis; complement activation. Disease: Complement Factor D Deficiency

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage