ELISA/assay

Bovine Pulmonary Surfactant Associated Protein A ELISA Kit

MBS018779

48-Strip-Wells

435 USD

ELISA Kit

Bovine Pulmonary Surfactant Associated Protein A ELISA Kit

Pulmonary Surfactant Associated Protein A

pulmonary surfactant-associated protein A; Pulmonary surfactant-associated protein A; pulmonary surfactant-associated protein A; PSAP; SP-A

SPA

SFTPA1; SFTPA1; PSP-A; SFTPA; SFTP1; SFTPA; PSAP; PSP-A; SP-A

SFTPA_PIG

Bovine

No significant cross-reactivity or interference between Bovine SPA and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 15.6 pg/ml - 500 pg/ml. Sensitivity: 2.0 pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SPA concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine SPA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SPA and SPA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SPA in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SPA concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine SPA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SPA and SPA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

47523604

NP_999430.1

NM_214265.1

P49874

26,702 Da

Pertussis Pathway (218109); Pertussis Pathway (218099); Phagosome Pathway (153902); Phagosome Pathway (153859)

Function: In presence of calcium ions, PSAP binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Subunit structure: Oligomeric complex of 6 set of homotrimers. Subcellular location: Secreted extracellular space extracellular matrix. Secreted extracellular space surface film. Miscellaneous: Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C). Sequence similarities: Belongs to the SFTPA family.Contains 1 C-type lectin domain.Contains 1 collagen-like domain.

48-Strip-Wells

435 USD

96-Strip-Wells

600