Human Leukocyte Antigen E ELISA Kit | MyBioSource MBS017623 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Leukocyte Antigen E ELISA Kit

catalog :

MBS017623

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS017623

products type :

ELISA Kit

products full name :

Human Leukocyte Antigen E ELISA Kit

products short name :

Leukocyte Antigen E

other names :

leukocyte antigen F; HLA class I histocompatibility antigen, alpha chain F; HLA class I histocompatibility antigen, alpha chain F; HLA F antigen; leukocyte antigen F; HLA class I molecule; MHC class Ib antigen; MHC class I antigen F; major histocompatibility complex, class I, F; CDA12; HLA F antigen; Leukocyte antigen F; MHC class I antigen F

products gene name :

HLA-E

other gene names :

HLA-F; HLA-F; HLAF; CDA12; HLA-5.4; HLA-CDA12; HLA-5.4; HLAF

uniprot entry name :

HLAF_HUMAN

reactivity :

Human

sequence length :

362

specificity :

No significant cross-reactivity or interference between Human HLA-E and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 3.12 ng/ml - 100 ng/ml. Sensitivity: 1.0 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated HLA-E concentrations in Human serum, plasma and other body fluids. Using Purified Human HLA-E antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add HLA-E and HLA-E antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HLA-E in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

products description :

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated HLA-E concentrations in Human serum, plasma and other body fluids. Using Purified Human HLA-E antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add HLA-E and HLA-E antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HLA-E in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

312407

ncbi acc num :

CAA34947.1

uniprot acc num :

P30511

ncbi mol weight :

39,062 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway (366163); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Antigen Processing-Cross Presentation Pathway (477122); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533)

ncbi summary :

This gene belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation. This gene exhibits few polymorphisms. Multiple transcript variants encoding different isoforms have been found for this gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain. [provided by RefSeq, Jul 2008]

uniprot summary :

HLA-F: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: Golgi membrane; phagocytic vesicle membrane; cell surface; membrane; endoplasmic reticulum; early endosome membrane; plasma membrane; MHC class I protein complex. Molecular Function: TAP1 binding; peptide antigen binding; TAP2 binding; receptor binding. Biological Process: antigen processing and presentation of peptide antigen via MHC class I; regulation of immune response; positive regulation of T cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage