catalog number :
MBS017592
products type :
ELISA Kit
products full name :
Bovine a1-Acid Glycoprotein ELISA Kit
products short name :
[a1-Acid Glycoprotein]
other names :
[alpha-1-acid glycoprotein-2, partial]
products gene name :
[a1-AGP]
specificity :
No significant cross-reactivity or interference between Bovine a1-AGP and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 31.2mug/ml-1000mug/ml. Sensitivity: 5.0mug/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated a1-AGP concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine a1-AGP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add a1-AGP and a1-AGP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of a1-AGP in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated a1-AGP concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine a1-AGP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add a1-AGP and a1-AGP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of a1-AGP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
AAA91124.1
size4 :
10x96-Strip-Wells