Canine Transferrin Receptor ELISA Kit | MyBioSource MBS017590 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Canine Transferrin Receptor ELISA Kit

catalog :

MBS017590

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS017590

products type :

ELISA Kit

products full name :

Canine Transferrin Receptor ELISA Kit

products short name :

Transferrin Receptor

other names :

transferrin receptor; Transferrin receptor protein 1; transferrin receptor protein 1; transferrin receptor (p90, CD71); T9; p90

products gene name :

TFR

other gene names :

TFRC; TFRC; T9; TR; TFR; p90; CD71; TFR1; TRFR; TR; TfR; TfR1; Trfr; sTfR

uniprot entry name :

TFR1_HUMAN

reactivity :

Canine

specificity :

No significant cross-reactivity or interference between Canine TFR and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 6.25 nmol/L - 200 nmol/L. Sensitivity: 1.0 nmol/L.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TFR concentrations in Canine serum, plasma and other body fluids. Using Purified Canine TFR antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TFR and TFR antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TFR in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

products description :

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TFR concentrations in Canine serum, plasma and other body fluids. Using Purified Canine TFR antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TFR and TFR antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TFR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

339516

ncbi acc num :

AAA61153.1

uniprot acc num :

P02786

ncbi mol weight :

84,871 Da

ncbi pathways :

Clathrin Derived Vesicle Budding Pathway (119545); Endocytosis Pathway (102279); Endocytosis Pathway (102181); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Golgi Associated Vesicle Biogenesis Pathway (119546); HIF-1 Signaling Pathway (695200); HIF-1-alpha Transcription Factor Network Pathway (138045); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Iron Metabolism In Placenta Pathway (672461)

uniprot summary :

TfR: the transferrin receptor. Regulates the cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied receptors into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Protein type: Cell surface; Receptor, misc.; Membrane protein, integral. Chromosomal Location of Human Ortholog: 3q29. Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; integral to plasma membrane; cytoplasmic membrane-bound vesicle; extracellular region; coated pit; recycling endosome; membrane; perinuclear region of cytoplasm; melanosome; plasma membrane; endosome; vesicle; external side of plasma membrane. Molecular Function: identical protein binding; protein binding; transferrin receptor activity; double-stranded RNA binding. Biological Process: receptor-mediated endocytosis; viral reproduction; cellular iron ion homeostasis; positive regulation of bone resorption; transferrin transport; osteoclast differentiation; regulation of cell growth; transmembrane transport; regulation of cell proliferation

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage