ELISA/assay

Horse Insulin Like Growth Factor1 ELISA Kit

MBS017382

96 Strip Wells

600 USD

ELISA Kit

Horse Insulin Like Growth Factor1 ELISA Kit

Insulin Like Growth Factor1

insulin-like growth factor 1 isoform 3 preproprotein; Insulin-like growth factor I; insulin-like growth factor 1; MGF; IGF-IA; IGF-IB; somatomedin-C; mechano growth factor; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; insulin-like growth factor 1 (somatomedin C); Mechano growth factor; MGF; Somatomedin-C

IGF1

IGF1; IGF1; IGFI; IGF-I; IGF1A; IBP1; IGF-I; MGF

IGF1_HUMAN

Horse

No significant cross-reactivity or interference between Horse IGF1 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IGF1 concentrations in Horse serum, plasma and other body fluids. Using Purified Horse IGF1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IGF1 and IGF1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IGF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Detection Range: The detection range of this kit is 15.6 ng/ml - 500 ng/ml. Sensitivity: The sensitivity of this kit is 2.0 ng/ml. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IGF1 concentrations in Horse serum, plasma and other body fluids. Using Purified Horse IGF1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IGF1 and IGF1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IGF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

163659903

NP_001104755.1

NM_001111285.1

P05019

21,841 Da

Adipogenesis Pathway 198832!!Aldosterone-regulated Sodium Reabsorption Pathway 130626!!Aldosterone-regulated Sodium Reabsorption Pathway 130590!!Apoptosis Pathway 198797!!Cardiac Progenitor Differentiation Pathway 712094!!Dilated Cardiomyopathy Pathway 121494!!Dilated Cardiomyopathy Pathway 121285!!Endochondral Ossification Pathway 198812!!Focal Adhesion Pathway 198795!!Focal Adhesion Pathway 83067

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]

Function: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. Ref.17. Subcellular location: Secreted. Involvement in disease: Insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]: Autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.Note: The disease is caused by mutations affecting the gene represented in this entry. Sequence similarities: Belongs to the insulin family. Sequence caution: The sequence CAA27250.1 differs from that shown. Reason: Erroneous gene model prediction.

96 Strip Wells

600 USD