Bovine Collagen Type X ELISA Kit | MyBioSource MBS017154 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Bovine Collagen Type X ELISA Kit

catalog :

MBS017154

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS017154

products type :

ELISA Kit

products full name :

Bovine Collagen Type X ELISA Kit

products short name :

Collagen Type X

other names :

collagen, type X, alpha 1(Schmid metaphyseal chondrodysplasia); Collagen alpha-1(X) chain; collagen alpha-1(X) chain; collagen alpha-1(X) chain; collagen X, alpha-1 polypeptide; Schmid metaphyseal chondrodysplasia; collagen, type X, alpha 1

products gene name :

COL10

other gene names :

COL10A1; COL10A1

uniprot entry name :

COAA1_HUMAN

reactivity :

Bovine

sequence length :

680

specificity :

This kit recognizes recombinant and natural Bovine COL10, no significant cross-reactivity or interference was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Detection Range: 3.12 ng/ml ~ 100 ng/ml. Sensitivity: 1.0 ng/ml.

other info2 :

Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Bovine COL10 in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Bovine COL10 concentration. The concentration of Bovine COL10 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) are less than 15%. Inter-assay Precision: Inter-assay CV (%) are less than 15%.

products description :

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample.

ncbi gi num :

119568625

ncbi acc num :

EAW48240.1

uniprot acc num :

Q03692

ncbi mol weight :

66,158 Da

ncbi pathways :

Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); Endochondral Ossification Pathway (198812); Extracellular Matrix Organization Pathway (576262); Senescence And Autophagy Pathway (198780)

ncbi summary :

This gene encodes the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer. Mutations in this gene are associated with Schmid type metaphyseal chondrodysplasia (SMCD) and Japanese type spondylometaphyseal dysplasia (SMD). [provided by RefSeq, Jul 2008]

uniprot summary :

COL10A1: Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage. Defects in COL10A1 are the cause of Schmid type metaphyseal chondrodysplasia (SMCD). SMCD is a dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 6q21-q22. Cellular Component: proteinaceous extracellular matrix; collagen; endoplasmic reticulum lumen; extracellular region; cell cortex. Molecular Function: metal ion binding. Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; cartilage development; skeletal development; endochondral ossification. Disease: Metaphyseal Chondrodysplasia, Schmid Type

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage