Monkey Granulocyte Colony Stimulating Factor ELISA Kit | MyBioSource MBS017118 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Monkey Granulocyte Colony Stimulating Factor ELISA Kit

catalog :

MBS017118

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS017118

products type :

ELISA Kit

products full name :

Monkey Granulocyte Colony Stimulating Factor ELISA Kit

products short name :

Granulocyte Colony Stimulating Factor

other names :

granulocyte colony-stimulating factor isoform d; Granulocyte colony-stimulating factor; granulocyte colony-stimulating factor; filgrastim; lenograstim; pluripoietin; colony stimulating factor 3 (granulocyte); Pluripoietin

products gene name :

GCSF

other gene names :

CSF3; CSF3; GCSF; CSF3OS; C17orf33; C17orf33; GCSF; G-CSF

uniprot entry name :

CSF3_HUMAN

reactivity :

Monkey

specificity :

No significant cross-reactivity or interference between Monkey GCSF and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 6.25 pg/ml - 200 pg/ml. Sensitivity: 1.0 pg/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GCSF concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey GCSF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GCSF and GCSF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GCSF in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

products description :

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GCSF concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey GCSF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GCSF and GCSF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GCSF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

296011057

ncbi acc num :

NP_001171618.1

ncbi gb acc num :

NM_001178147.1

uniprot acc num :

P09919

ncbi mol weight :

22,293 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Malaria Pathway (152665); Malaria Pathway (152657); PI3K-Akt Signaling Pathway (692234)

ncbi summary :

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

uniprot summary :

G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Cell cycle regulation; Cytokine; Secreted. Chromosomal Location of Human Ortholog: 17q11.2-q12. Cellular Component: extracellular space. Molecular Function: enzyme binding; growth factor activity; cytokine activity; granulocyte colony-stimulating factor receptor binding. Biological Process: positive regulation of protein binding; positive regulation of myeloid cell differentiation; granulocyte differentiation; multicellular organismal development; cytokine and chemokine mediated signaling pathway; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of actin filament polymerization; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; immune response

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage