ELISA/assay

Rat Tissue Factor ELISA Kit

MBS017018

96 Strip Wells

600 USD

ELISA Kit

Rat Tissue Factor ELISA Kit

Tissue Factor

tissue factor; Tissue factor; tissue factor; coagulation factor III (thromboplastin, tissue factor); Coagulation factor III; Thromboplastin

TF

F3; F3; TF; TFA; CD142; TF

TF_HUMAN

Rat

No significant cross-reactivity or interference between Rat TF and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TF concentrations in Rat serum, plasma and other body fluids. Using Purified Rat TF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TF and TF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TF in the samples is then determined by comparing the O.D. of the samples to the standard curve. Detection Range: The detection range of this kit is 15.6 pg/ml – 500 pg/ml.

Sensitivity: The sensitivity of this kit is 2.0 pg/ml. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TF concentrations in Rat serum, plasma and other body fluids. Using Purified Rat TF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TF and TF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

339508

AAA36734.1

P13726

33,068 Da

Complement And Coagulation Cascades Pathway 198880!!Complement And Coagulation Cascades Pathway 83073!!Complement And Coagulation Cascades Pathway 484!!Extrinsic Pathway 106058!!Formation Of Fibrin Clot (Clotting Cascade) Pathway 106057!!Hemostasis Pathway 106028

This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Function: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. Ref.6. Subunit structure: Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII. Ref.15. Subcellular location: Isoform 1: Membrane; Single-pass type I membrane protein Ref.6. Isoform 2: Secreted Ref.6. Tissue specificity: Lung, placenta and pancreas. Ref.6. Induction: TF expression is highly dependent upon cell type. TF can also be induced by the inflammatory mediators interleukin 1 and TNF-alpha, as well as by endotoxin, to appear on monocytes and vascular endothelial cells as a component of cellular immune response. Ref.14. Sequence similarities: Belongs to the tissue factor family.

96 Strip Wells

600 USD