ELISA/assay

Mouse Arrestin Beta 1 ELISA Kit

MBS016953

48-Strip-Wells

435 USD

ELISA Kit

Mouse Arrestin Beta 1 ELISA Kit

Arrestin Beta 1

arrestin beta 1 transcript variant 2; Beta-arrestin-1; beta-arrestin-1; arrestin 2; arrestin, beta 1; Arrestin beta-1

ARRB1

ARRB1; ARRB1; ARB1; ARR1; ARR1

ARRB1_HUMAN

Mouse

410

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma and Tissue Homogenate. Assay Type: Sandwich. Detection Range: 31.2 pg/ml - 1000 pg/ml. Sensitivity: 5.0 pg/ml.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of ARRB1 (hereafter termed this analyte) in undiluted original Mouse serum, plasma and tissue homogenate samples.

209944101

ACI96306.1

P49407

47,066 Da

Activated NOTCH1 Transmits Signal To The Nucleus Pathway (576270); CXCR3-mediated Signaling Events Pathway (138011); Calcium Regulation In The Cardiac Cell Pathway (198906); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Clathrin Derived Vesicle Budding Pathway (119545); Corticotropin-releasing Hormone Pathway (920957); Disease Pathway (530764); Endocytosis Pathway (102279); Endocytosis Pathway (102181)

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described. [provided by RefSeq, Jan 2011]

ARRB1: regulates G-protein coupled receptors (GPCR) signaling by mediating both receptor desensitization and resensitization processes. Binds to GRK-phosphorylated receptor and sterically preclude its coupling to the cognate G- protein; the binding appears to require receptor determinants exposed only in the active receptor conformation. Targets many receptors for internalization by acting as endocytic adapters (CLASPs, clathrin-associated sorting proteins). Internalized arrestin-receptor complexes traffic to intracellular endosomes, where they remain uncoupled from G-proteins. Two different modes of arrestin-mediated internalization occur. Beta-arrestins function as multivalent adapter proteins that can switch the GPCR from a G-protein signaling mode that transmits short-lived signals from the plasma membrane via small molecule second messengers and ion channels to a beta-arrestin signaling mode that transmits a distinct set of signals that are initiated as the receptor internalizes and transits the intracellular compartment. Also involved in regulation of receptors other than GPCRs. Involved in Toll-like receptor and IL-1 receptor signaling through the interaction with TRAF6 which prevents TRAF6 autoubiquitination and oligomerization required for activation of NF-kappa-B and JUN. Binds phosphoinositides. Binds inositolhexakisphosphate (InsP6). Involved in IL8-mediated granule release in neutrophils. Interacts with phosphorylated ADRB2 and CHRM2. Interacts with SRC (via the SH3 domain and the protein kinase domain); the interaction is independent of the phosphorylation state of SRC C-terminus. Interacts with RAF1, CHUK, IKBKB and Nik. Interacts with DVL1 and DVL2; the interaction is enhanced by DVL phosphorylation. Interacts with IGF1R. Belongs to the arrestin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 11q13. Cellular Component: cytoplasmic vesicle membrane; lysosomal membrane; coated pit; pseudopodium; cytosol; Golgi membrane; nucleoplasm; cytoplasm; plasma membrane; heterotrimeric G-protein complex; cytoplasmic vesicle; nucleus; chromatin. Molecular Function: angiotensin receptor binding; enzyme inhibitor activity; insulin-like growth factor receptor binding; protein binding; histone acetyltransferase activity; mitogen-activated protein kinase kinase binding; ubiquitin protein ligase binding; caspase inhibitor activity; transcription factor binding; GTPase activator activity. Biological Process: transcription from RNA polymerase II promoter; platelet activation; proteasomal ubiquitin-dependent protein catabolic process; Notch signaling pathway; positive regulation of protein binding; positive regulation of receptor internalization; protein ubiquitination; positive regulation of Rho protein signal transduction; negative regulation of caspase activity; positive regulation of peptidyl-serine phosphorylation; protein transport; G-protein coupled receptor internalization; inhibition of NF-kappaB transcription factor; negative regulation of interleukin-6 production; stress fiber formation; negative regulation of interleukin-8 production; phototransduction; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein ubiquitination; post-Golgi vesicle-mediated transport; positive regulation of protein amino acid phosphorylation; blood coagulation; positive regulation of GTPase activity

48-Strip-Wells

435 USD

96-Strip-Wells

600