Fish C Reactive Protein ELISA Kit | MyBioSource MBS016586 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Fish C Reactive Protein ELISA Kit

catalog :

MBS016586

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS016586

products type :

ELISA Kit

products full name :

Fish C Reactive Protein ELISA Kit

products short name :

C Reactive Protein

other names :

C-reactive protein; C-reactive protein; C-reactive protein; pentraxin 1; C-reactive protein, pentraxin-related

products gene name :

CRP

other gene names :

CRP; CRP; PTX1; PTX1

uniprot entry name :

CRP_HUMAN

reactivity :

Fish

specificity :

No significant cross-reactivity or interference between Fish CRP and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.25 mug/ml - 8 mug/ml. Sensitivity: 0.1 mug/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CRP concentrations in Fish serum, plasma and other body fluids. Using Purified Fish CRP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CRP and CRP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CRP in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated CRP concentrations in Fish serum, plasma and other body fluids. Using Purified Fish CRP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CRP and CRP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CRP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

55770842

ncbi acc num :

NP_000558.2

ncbi gb acc num :

NM_000567.2

uniprot acc num :

P02741

ncbi mol weight :

25,039 Da

ncbi pathways :

Classical Antibody-mediated Complement Activation Pathway (106409); Complement Cascade Pathway (106405); Creation Of C4 And C2 Activators Pathway (106407); IL6-mediated Signaling Events Pathway (137932); Immune System Pathway (106386); Initial Triggering Of Complement Pathway (106406); Innate Immune System Pathway (106387); Selenium Pathway (198825)

ncbi summary :

The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. [provided by RefSeq, Sep 2009]

uniprot summary :

CRP: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. Belongs to the pentaxin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q23.2. Cellular Component: extracellular space; growth cone; extracellular region; filopodium. Molecular Function: choline binding; protein binding; protein homodimerization activity; low-density lipoprotein receptor binding; complement component C1q binding; cholesterol binding; virion binding; low-density lipoprotein binding; calcium ion binding. Biological Process: protein polymerization; defense response to Gram-positive bacterium; response to ethanol; wound healing; negative regulation of vasodilation; positive regulation of superoxide release; response to lead ion; acute-phase response; response to hypoxia; opsonization; inflammatory response; complement activation, classical pathway; aging

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage