ELISA/assay

Rat Matrix Metalloproteinase 3 ELISA Kit

MBS016574

48-Strip-Wells

435 USD

ELISA Kit

Rat Matrix Metalloproteinase 3 ELISA Kit

Matrix Metalloproteinase 3

MMP-3; stromelysin-1; metalloproteinase 3 receptor; matrix metallopeptidase 3 (stromelysin 1, progelatinase)

MMP-3

MMP3

Rat

No significant cross-reactivity or interference between Rat MMP-3 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 3.12 ng/ml - 100 ng/ml. Sensitivity: 1.0 ng/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MMP-3 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat MMP-3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MMP-3 and MMP-3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MMP-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MMP-3 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat MMP-3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MMP-3 and MMP-3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MMP-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

8176609

BAA96388.1

Matrix Metalloproteinases Pathway (198565); Rheumatoid Arthritis Pathway (200308); Rheumatoid Arthritis Pathway (200269); TNF Signaling Pathway (812258); TNF Signaling Pathway (813210); Transcriptional Misregulation In Cancer Pathway (522997); Transcriptional Misregulation In Cancer Pathway (522987)

48-Strip-Wells

435 USD

96-Strip-Wells

600