Human Defensin 1, Neutrophil ELISA Kit | MyBioSource MBS016501 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Defensin 1, Neutrophil ELISA Kit

catalog :

MBS016501

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS016501

products type :

ELISA Kit

products full name :

Human Defensin 1, Neutrophil ELISA Kit

products short name :

Defensin 1, Neutrophil

other names :

Defensin, alpha 1, preproprotein; Neutrophil defensin 1; neutrophil defensin 1; defensin, alpha 2; myeloid-related sequence; defensin, alpha 1, myeloid-related sequence; defensin, alpha 1; Defensin, alpha 1; HNP-1; HP-1; HP1Cleaved into the following 2 chains:HP 1-56Neutrophil defensin 2; Alternative name(s):; HNP-2

products gene name :

DEFA1

other gene names :

DEFA1; DEFA1; HP1; MRS; DEF1; HP-1; DEFA2; HNP-1; DEF1; DEFA2; MRS; HP-1; HP1; HP-2; HP2

uniprot entry name :

DEF1_HUMAN

reactivity :

Human

sequence length :

specificity :

No significant cross-reactivity or interference between Human DEFA1 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 31.2 pg/ml - 1000 pg/ml. Sensitivity: 5.0 pg/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated DEFA1 concentrations in Human serum, plasma and other body fluids. Using Purified Human DEFA1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add DEFA1 and DEFA1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DEFA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated DEFA1 concentrations in Human serum, plasma and other body fluids. Using Purified Human DEFA1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add DEFA1 and DEFA1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DEFA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

85567619

ncbi acc num :

AAI12189.1

uniprot acc num :

P59665

ncbi mol weight :

10,201 Da

ncbi pathways :

Alpha-defensins Pathway (530758); Cellular Roles Of Anthrax Toxin Pathway (138076); Defensins Pathway (530757); Immune System Pathway (106386); Innate Immune System Pathway (106387)

ncbi summary :

Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. [provided by RefSeq, May 2010]

uniprot summary :

defensin, alpha 1: Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram- negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Belongs to the alpha-defensin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 8p23.1. Cellular Component: extracellular matrix; extracellular space; Golgi lumen; extracellular region. Biological Process: defense response to Gram-positive bacterium; estrogen receptor signaling pathway; innate immune response in mucosa; killing of cells of another organism; antibacterial humoral response; innate immune response; immune response; chemotaxis; defense response to virus; defense response to fungus

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage