ELISA/assay

Porcine Tartrate Resistant Acid Phosphatase 5B ELISA Kit

MBS015541

48-Strip-Wells

435 USD

ELISA Kit

Porcine Tartrate Resistant Acid Phosphatase 5B ELISA Kit

Tartrate Resistant Acid Phosphatase 5B

tartrate-resistant acid phosphatase type 5; Tartrate-resistant acid phosphatase type 5; tartrate-resistant acid phosphatase type 5; TrATPase; tartrate-resistant acid ATPase; acid phosphatase 5, tartrate resistant; Tartrate-resistant acid ATPase; TrATPase; Type 5 acid phosphatase

TRACP-5B

ACP5; ACP5; TRAP; SPENCDI; TR-AP; TrATPase

PPA5_HUMAN

Porcine

325

No significant cross-reactivity or interference between Porcine TRACP-5B and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 0.5 U/L - 16 U/L. Sensitivity: 0.1 U/L.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TRACP-5B concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine TRACP-5B antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TRACP-5B and TRACP-5B antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TRACP-5B in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated TRACP-5B concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine TRACP-5B antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TRACP-5B and TRACP-5B antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TRACP-5B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

161377455

NP_001104506.1

NM_001111036.1

P13686

36,599 Da

Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (906000); Defective BTD Causes Biotidinase Deficiency Pathway (906015); Defective CD320 Causes Methylmalonic Aciduria Pathway (906012); Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway (906001); Defective GIF Causes Intrinsic Factor Deficiency Pathway (906004); Defective HLCS Causes Multiple Carboxylase Deficiency Pathway (906014); Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway (906003); Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway (906010); Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway (906009); Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway (906005)

This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq, Aug 2008]

ACP5: Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. Defects in ACP5 are the cause of spondyloenchondrodysplasia with immune dysregulation (SPENCDI). A disease characterized by vertebral and metaphyseal dysplasia, spasticity with cerebral calcifications, and strong predisposition to autoimmune diseases. The skeletal dysplasia is characterized by radiolucent and irregular spondylar and metaphyseal lesions that represent islands of chondroid tissue within bone. ACP5 inactivating mutations result in a functional excess of phosphorylated osteopontin causing deregulation of osteopontin signaling and consequential autoimmune disease. Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family. Protein type: EC 3.1.3.2; Phosphatase; Motility/polarity/chemotaxis; Cofactor and Vitamin Metabolism - riboflavin. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: lysosome; integral to membrane; cytosol. Molecular Function: acid phosphatase activity; ferric iron binding; ferrous iron binding. Biological Process: negative regulation of interleukin-12 production; vitamin metabolic process; negative regulation of nitric oxide biosynthetic process; response to lipopolysaccharide; negative regulation of superoxide release; negative regulation of interleukin-1 beta production; negative regulation of tumor necrosis factor production; riboflavin metabolic process; defense response to Gram-positive bacterium; dephosphorylation; negative regulation of inflammatory response; response to cytokine stimulus; water-soluble vitamin metabolic process; bone resorption. Disease: Spondyloenchondrodysplasia With Immune Dysregulation

48-Strip-Wells

435 USD

96-Strip-Wells

600