Fish Phospho-Nuclear Factor Kappa B ELISA Kit | MyBioSource MBS015059 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Fish Phospho-Nuclear Factor Kappa B ELISA Kit

catalog :

MBS015059

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS015059

products type :

ELISA Kit

products full name :

Fish Phospho-Nuclear Factor Kappa B ELISA Kit

products short name :

Phospho-Nuclear Factor Kappa B

other names :

protein phosphatase 1 regulatory subunit 14A isoform 2; Protein phosphatase 1 regulatory subunit 14A; protein phosphatase 1 regulatory subunit 14A; 17-KDa protein; PKC-potentiated inhibitory protein of PP1; 17 kDa PKC-potentiated inhibitory protein of PP1; 17-kDa PKC-potentiated inhibitory protein of PP1; protein kinase C-potentiated inhibitor protein of 17 kDa; protein phosphatase 1, regulatory (inhibitor) subunit 14A; 17 kDa PKC-potentiated inhibitory protein of PP1; Protein kinase C-potentiated inhibitor protein of 17 kDa

products gene name :

PNFKB

other gene names :

PPP1R14A; PPP1R14A; CPI17; CPI-17; PPP1INL; CPI17; PPP1INL; CPI-17

uniprot entry name :

PP14A_HUMAN

reactivity :

Fish

sequence length :

120

specificity :

No significant cross-reactivity or interference between Fish PNFKB and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 62.5 pg/ml - 2000 pg/ml. Sensitivity: 10 pg/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PNFKB concentrations in Fish serum, plasma and other body fluids. Using Purified Fish PNFKB antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PNFKB and PNFKB antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PNFKB in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

products description :

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PNFKB concentrations in Fish serum, plasma and other body fluids. Using Purified Fish PNFKB antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PNFKB and PNFKB antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PNFKB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

345441807

ncbi acc num :

NP_001230876.1

ncbi gb acc num :

NM_001243947.1

uniprot acc num :

Q96A00

ncbi mol weight :

16,693 Da

ncbi pathways :

Vascular Smooth Muscle Contraction Pathway (96530); Vascular Smooth Muscle Contraction Pathway (96866)

ncbi summary :

The protein encoded by this gene belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Sep 2011]

uniprot summary :

PPP1R14A: a phosphorylation-dependent inhibitory subunit of protein phosphatase 1 (PP-1A). Phosphorylation by PKC regulates its binding to PP-1A. Acts co-operatively to inhibit myosin light chain phosphatase (MLCP) activity. Inhibition leads to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2 ) concentration. Involved in the regulation of cell morphology, re-organisation of microfilaments, and cell spreading. Also expressed in human platelets and in brain where it may play a role in cerebellar long-term synaptic depression. Phosphorylation by ILK or Rho kinase enhances its ability to inhibit PP-1A. Protein type: Inhibitor; Protein phosphatase, regulatory subunit. Chromosomal Location of Human Ortholog: 19q13.1. Cellular Component: cytoplasm. Molecular Function: protein phosphatase inhibitor activity. Biological Process: regulation of phosphorylation; negative regulation of catalytic activity

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage