Horse N-Terminal Pro-Brain Natriuretic Peptide ELISA Kit | MyBioSource MBS014699 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Horse N-Terminal Pro-Brain Natriuretic Peptide ELISA Kit

catalog :

MBS014699

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS014699

products type :

ELISA Kit

products full name :

Horse N-Terminal Pro-Brain Natriuretic Peptide ELISA Kit

products short name :

N-Terminal Pro-Brain Natriuretic Peptide

other names :

natriuretic peptides B preproprotein; Natriuretic peptides B; natriuretic peptides B; natriuretic protein; brain type natriuretic peptide; gamma-brain natriuretic peptide; natriuretic peptide precursor B; natriuretic peptide B; Gamma-brain natriuretic peptide

products gene name :

NT-PROBNP

other gene names :

NPPB; NPPB; BNP; BNP(1-32); BNP-32

uniprot entry name :

ANFB_HUMAN

reactivity :

Horse

sequence length :

134

specificity :

No significant cross-reactivity or interference between Horse NT-PROBNP and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids. Assay Type: Sandwich. Detection Range: 31.2 pg/ml - 1000 pg/ml. Sensitivity: 5.0 pg/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NT-PROBNP concentrations in Horse serum, plasma and other body fluids. Using Purified Horse NT-PROBNP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NT-PROBNP and NT-PROBNP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NT-PROBNP in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NT-PROBNP concentrations in Horse serum, plasma and other body fluids. Using Purified Horse NT-PROBNP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NT-PROBNP and NT-PROBNP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NT-PROBNP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

4505433

ncbi acc num :

NP_002512.1

ncbi gb acc num :

NM_002521.2

uniprot acc num :

P16860

ncbi mol weight :

14,726 Da

ncbi pathways :

MicroRNAs In Cardiomyocyte Hypertrophy Pathway (198784); CGMP-PKG Signaling Pathway (983748); CGMP-PKG Signaling Pathway (985584)

ncbi summary :

This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq, Jul 2008]

uniprot summary :

NPPB: Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Belongs to the natriuretic peptide family. Protein type: Hormone; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1p36.2. Cellular Component: extracellular space; protein complex; extracellular region. Molecular Function: diuretic hormone activity; hormone activity; receptor binding. Biological Process: cGMP biosynthetic process; negative regulation of angiogenesis; cell surface receptor linked signal transduction; regulation of vasodilation; regulation of blood pressure; receptor guanylyl cyclase signaling pathway; negative regulation of cell growth; regulation of vascular permeability; regulation of blood vessel size; body fluid secretion

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage