Sheep Heat Shock Protein 27 ELISA Kit | MyBioSource MBS014395 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Sheep Heat Shock Protein 27 ELISA Kit

catalog :

MBS014395

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS014395

products type :

ELISA Kit

products full name :

Sheep Heat Shock Protein 27 ELISA Kit

products short name :

Heat Shock Protein 27

other names :

HSP27; Heat shock protein beta-1; heat shock protein beta-1; HSP 27; 28 kDa heat shock protein; heat shock 27 kDa protein; heat shock 27kD protein 1; stress-responsive protein 27; estrogen-regulated 24 kDa protein; heat shock 27kDa protein 1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27

products gene name :

HSP27

other gene names :

HSPB1; HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HSP27; HSP28; HspB1; HSP 27; SRP27

uniprot entry name :

HSPB1_HUMAN

reactivity :

Sheep

specificity :

No significant cross-reactivity or interference between Sheep HSP27 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 62.5 pg/ml - 2000 pg/ml. Sensitivity: 10 pg/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated HSP27 concentrations in Sheep serum, plasma and other body fluids. Using Purified Sheep HSP27 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add HSP27 and HSP27 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HSP27 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated HSP27 concentrations in Sheep serum, plasma and other body fluids. Using Purified Sheep HSP27 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add HSP27 and HSP27 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HSP27 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

11036357

ncbi acc num :

BAB17232.1

uniprot acc num :

P04792

ncbi mol weight :

22,783 Da

ncbi pathways :

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Destabilization Of MRNA By AUF1 (hnRNP D0) Pathway (187212); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); FAS Pathway And Stress Induction Of HSP Regulation (198894); Gene Expression Pathway (105937); IL-3 Signaling Pathway (198881); IL-6 Signaling Pathway (198864); MAPK Signaling Pathway (198779)

ncbi summary :

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]

uniprot summary :

HSP27: a small heat shock protein that is regulated both transcriptionally and posttranslationally. Modulates actin polymerization and reorganization. Its expression level increases several-fold in response to stress and is phosphorylated by MAPKAP kinase 2. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock. Protein type: Motility/polarity/chemotaxis; Heat shock protein; Chaperone. Chromosomal Location of Human Ortholog: 7q11.23. Cellular Component: proteasome complex; extracellular space; focal adhesion; cytoskeleton; cytoplasm; plasma membrane; spindle; cytosol; Z disc; nucleus. Molecular Function: identical protein binding; protein kinase C inhibitor activity; protein binding; ubiquitin binding; protein kinase C binding; protein kinase binding. Biological Process: positive regulation of tumor necrosis factor biosynthetic process; response to virus; positive regulation of blood vessel endothelial cell migration; response to unfolded protein; positive regulation of interleukin-1 beta production; retinal homeostasis; positive regulation of angiogenesis; negative regulation of protein kinase activity; gene expression; cell motility; regulation of I-kappaB kinase/NF-kappaB cascade; vascular endothelial growth factor receptor signaling pathway; negative regulation of apoptosis; regulation of translational initiation. Disease: Neuronopathy, Distal Hereditary Motor, Type Iib; Charcot-marie-tooth Disease, Axonal, Type 2f

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage