ELISA/assay

Rat Macrophage Migration Inhibitory Factor ELISA Kit

MBS014165

96 Strip Wells

600 USD

ELISA Kit

Rat Macrophage Migration Inhibitory Factor ELISA Kit

Macrophage Migration Inhibitory Factor

macrophage migration inhibitory factor; Macrophage migration inhibitory factor; macrophage migration inhibitory factor; L-dopachrome isomerase; L-dopachrome tautomerase; phenylpyruvate tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor); Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase (EC:5.3.3.12); Phenylpyruvate tautomerase

MIF

MIF; MIF; GIF; GLIF; MMIF; GLIF; MMIF; MIF; GIF

MIF_HUMAN

Rat

No significant cross-reactivity or interference between Rat MIF and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MIF concentrations in Rat serum, plasma and other body fluids. Using Purified Rat MIF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MIF and MIF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MIF in the samples is then determined by comparing the O.D. of the samples to the standard curve. Detection Range: The detection range of this kit is 3.12ng/ml-100ng/ml.

Sensitivity: The sensitivity of this kit is 1.0ng/ml. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MIF concentrations in Rat serum, plasma and other body fluids. Using Purified Rat MIF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MIF and MIF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MIF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4505185

NP_002406.1

NM_002415.1

P14174

12,476 Da

Adipogenesis Pathway 198832!!Phenylalanine Metabolism Pathway 82960!!Phenylalanine Metabolism Pathway 327!!Spinal Cord Injury Pathway 739007!!Tyrosine Metabolism Pathway 82959!!Tyrosine Metabolism Pathway 325

This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]

Function: Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. Ref.23 Ref.24. Catalytic activity: Keto-phenylpyruvate = enol-phenylpyruvate. Ref.32 Ref.34L-dopachrome = 5,6-dihydroxyindole-2-carboxylate. Ref.32 Ref.34. Subunit structure: Homotrimer. Interacts with CXCR2 extracellular domain . By similarity. Interacts with the CD74 extracellular domain, COPS5 and BNIPL. Ref.18 Ref.21 Ref.22 Ref.25 Ref.33 Ref.34. Subcellular location: Secreted. Cytoplasm. Note: Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens. Ref.1 Ref.2 Ref.18 Ref.23 Ref.25. Induction: Up-regulated in concanavalin-A-treated lymphocytes. Up-regulated in macrophages upon exposure to M.tuberculosis antigens. Ref.1 Ref.23. Involvement in disease: Rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]: An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Miscellaneous: Serum levels of MIF are elevated in patients with severe sepsis or septic shock. High levels of MIF are correlated with low survival. Drugs that inhibit tautomerase activity protect against death due to sepsis. Sequence similarities: Belongs to the MIF family. Biophysicochemical propertiesKinetic parameters:KM=249 uM for phenylpyruvate Ref.20KM=168 uM for p-hydroxyphenylpyruvateVmax=2113 umol/min/mg enzyme toward phenylpyruvateVmax=524 umol/min/mg enzyme toward p-hydroxyphenylpyruvate

96 Strip Wells

600 USD